RRC ID 37583
Author Hou C, Meng F, Zhang Z, Kang Y, Chen W, Huang G, Fu M, Sheng P, Zhang Z, Liao W.
Title The Role of MicroRNA-381 in Chondrogenesis and Interleukin-1-β Induced Chondrocyte Responses.
Journal Cell Physiol Biochem
Abstract AIM:The molecular pathways regulating cartilage degradation are unclear. miR-381 was identified as a putative regulator of chondrogenesis related genes. Here, we examined its role in chondrogenesis and osteoarthritic cartilage degeneration.
METHODS:miR-381 expression was assessed in vitro in response to IL-1β stimulation in primary human (PHC) and mouse (PMC) chondrocytes, and ATDC5 derived chondrocytes; and in vivo in mouse embryos and human osteoarthritic cartilage. The effects of miR-381 on chondrogenesis and NF-kB signaling were assessed using a synthetic RNA mimic or inhibitor and luciferase assay, respectively. Upstream regulators of miR381 were probed using siRNA or overexpression plasmids for Sox9 and Runx2.
RESULTS:miR-381 expression was elevated in chondrogenic and hypertrophic ATDC5 cells. miR-381 was induced in vitro by IL-1β in ATDC5 cells, PMCs, and PHCs, and was expressed in areas of cartilage degradation or absorption in vivo. Overexpression of Runx2 or Sox9 increased miR-381 expression in ATDC5 cells. miR-381 suppressed expression of collagen, type II, alpha 1, and enhanced expression of metalloproteinase-13 (MMP-13), but did not regulate NFKBIA and NKRF activity.
CONCLUSION:miR-381 was highly expressed during chondrogenesis and in arthritic cartilage. It may contribute to absorption of the cartilage matrix by repressing type II collagen and inducing MMP-13.
Volume 36(5)
Pages 1753-66
Published 2015-1-1
DOI 10.1159/000430148
PII 000430148
PMID 26184031
MeSH Animals Arthritis / genetics Arthritis / pathology Cell Line Chondrocytes / cytology* Chondrogenesis / genetics* Collagen Type II / metabolism Core Binding Factor Alpha 1 Subunit / metabolism Humans Interleukin-1beta / physiology* Matrix Metalloproteinase 13 / metabolism Mice MicroRNAs / physiology* SOX9 Transcription Factor / metabolism
IF 5.5
Times Cited 31
Human and Animal Cells ATDC5(RCB0565)