論文 - 詳細
RRC ID | 37587 |
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著者 | Taniguchi Y, Kakura K, Yamamoto K, Kido H, Yamazaki J. |
タイトル | Accelerated Osteogenic Differentiation and Bone Formation on Zirconia with Surface Grooves Created with Fiber Laser Irradiation. |
ジャーナル | Clin Implant Dent Relat Res |
Abstract |
BACKGROUND:Modification of the surface topography of biomaterials is a critical factor for the proliferation and differentiation of osteoblasts. Elucidating the biological response to surface roughening is necessary for clinical application of zirconia implants. PURPOSE:To investigate the effects of fiber laser-induced zirconia surface roughening on cultured osteoblast-like cell morphology, proliferation, differentiation, and calcification, and on in vivo bone formation. MATERIALS AND METHODS:Sixty-six machine-surfaced yttria-tetragonal zirconia polycrystal plates (S-Zr) and 16 machine-surfaced implants were used as controls. We prepared 66 rough plates (R-Zr) and 16 rough implants by surface treatment using a fiber laser. RESULTS:MC3T3-E1 cells spread well in all directions on S-Zr, whereas elongated cells with poorly organized actin stress fibers were observed on R-Zr. Cell proliferation was significantly greater on R-Zr than on S-Zr. The Runx2 mRNA level increased time dependently in osteogenic culture condition. Alkaline phosphatase activity and osteocalcin mRNA levels were higher on R-Zr compared with S-Zr. Alizarin red S staining revealed greater calcification on R-Zr than on S-Zr. Laser treatment of zirconia implant bodies placed in rat tibiae increased the bone-implant contact ratio and removal torque considerably. CONCLUSIONS:Our results suggest that fiber laser irradiation produces adequate surface roughening of zirconia ceramics to support osseointegration. |
巻・号 | 18(5) |
ページ | 883-894 |
公開日 | 2016-10-1 |
DOI | 10.1111/cid.12366 |
PMID | 26179832 |
MeSH | Animals In Vitro Techniques Lasers* Male Osteogenesis / physiology* Rats Rats, Sprague-Dawley Surface Properties Zirconium* |
IF | 3.396 |
引用数 | 8 |
WOS 分野 | DENTISTRY, ORAL SURGERY & MEDICINE |
リソース情報 | |
ヒト・動物細胞 | MC3T3-E1(RCB1126) |