論文 - 詳細
| RRC ID | 37602 |
|---|---|
| 著者 | Minato A, Ise H, Goto M, Akaike T. |
| タイトル | Cardiac differentiation of embryonic stem cells by substrate immobilization of insulin-like growth factor binding protein 4 with elastin-like polypeptides. |
| ジャーナル | Biomaterials |
| Abstract |
The establishment of cardiomyocyte differentiation of embryonic stem cells (ESCs) is a useful strategy for cardiovascular regenerative medicine. Here, we report a strategy for cardiomyocyte differentiation of ESCs using substrate immobilization of insulin-like growth factor binding protein 4 (IGFBP4) with elastin-like polypeptides. Recently, IGFBP4 was reported to promote cardiomyocyte differentiation of ESCs through inhibition of the Wnt/β-catenin signaling. However, high amounts of IGFBP4 (approximately 1 μg/mL) were required to inhibit the Wnt/β-catenin signaling and induce differentiation to cardiomyocytes. We report herein induction of cardiomyocyte differentiation using IGFBP4-immobilized substrates. IGFBP4-immobilized substrates were created by fusion with elastin-like polypeptides. IGFBP4 was stably immobilized to polystyrene dishes through fusion of elastin-like polypeptides. Cardiomyocyte differentiation of ESCs was effectively promoted by strong and continuous inhibition of Wnt/β-catenin signaling with IGFBP4-immobilized substrates. These results demonstrated that IGFBP4 could be immobilized using fusion of elastin-like polypeptides. Our results also demonstrate that substrate immobilization of IGFBP4 is a powerful tool for differentiation of ESCs into cardiomyocytes. These findings suggest that substrate immobilization of soluble factors is a useful technique for differentiation of ESCs in regenerative medicine and tissue engineering. |
| 巻・号 | 33(2) |
| ページ | 515-23 |
| 公開日 | 2012-1-1 |
| DOI | 10.1016/j.biomaterials.2011.09.070 |
| PII | S0142-9612(11)01146-X |
| PMID | 22018385 |
| MeSH | Animals Biopolymers / metabolism Blotting, Western Cell Differentiation* Cell Line, Tumor Elastin / chemistry Embryonic Stem Cells / cytology* Immobilized Proteins / chemistry* Immunoprecipitation Insulin-Like Growth Factor Binding Protein 4 / chemistry* Mice Mice, Inbred C3H Myocytes, Cardiac / cytology* Peptides / metabolism Plasmids Recombinant Proteins Signal Transduction Tissue Engineering Wnt Proteins / antagonists & inhibitors Wnt Proteins / genetics Wnt Proteins / metabolism beta Catenin / antagonists & inhibitors beta Catenin / genetics beta Catenin / metabolism |
| IF | 3.978 |
| 引用数 | 8 |
| WOS 分野 | CHEMISTRY, ANALYTICAL |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 各媒体での言及数の合計 | 0 |
| リソース情報 | |
| ヒト・動物細胞 | P19CL6 |