| RRC ID |
37602
|
| Author |
Minato A, Ise H, Goto M, Akaike T.
|
| Title |
Cardiac differentiation of embryonic stem cells by substrate immobilization of insulin-like growth factor binding protein 4 with elastin-like polypeptides.
|
| Journal |
Biomaterials
|
| Abstract |
The establishment of cardiomyocyte differentiation of embryonic stem cells (ESCs) is a useful strategy for cardiovascular regenerative medicine. Here, we report a strategy for cardiomyocyte differentiation of ESCs using substrate immobilization of insulin-like growth factor binding protein 4 (IGFBP4) with elastin-like polypeptides. Recently, IGFBP4 was reported to promote cardiomyocyte differentiation of ESCs through inhibition of the Wnt/β-catenin signaling. However, high amounts of IGFBP4 (approximately 1 μg/mL) were required to inhibit the Wnt/β-catenin signaling and induce differentiation to cardiomyocytes. We report herein induction of cardiomyocyte differentiation using IGFBP4-immobilized substrates. IGFBP4-immobilized substrates were created by fusion with elastin-like polypeptides. IGFBP4 was stably immobilized to polystyrene dishes through fusion of elastin-like polypeptides. Cardiomyocyte differentiation of ESCs was effectively promoted by strong and continuous inhibition of Wnt/β-catenin signaling with IGFBP4-immobilized substrates. These results demonstrated that IGFBP4 could be immobilized using fusion of elastin-like polypeptides. Our results also demonstrate that substrate immobilization of IGFBP4 is a powerful tool for differentiation of ESCs into cardiomyocytes. These findings suggest that substrate immobilization of soluble factors is a useful technique for differentiation of ESCs in regenerative medicine and tissue engineering.
|
| Volume |
33(2)
|
| Pages |
515-23
|
| Published |
2012-1-1
|
| DOI |
10.1016/j.biomaterials.2011.09.070
|
| PII |
S0142-9612(11)01146-X
|
| PMID |
22018385
|
| MeSH |
Animals
Biopolymers / metabolism
Blotting, Western
Cell Differentiation*
Cell Line, Tumor
Elastin / chemistry
Embryonic Stem Cells / cytology*
Immobilized Proteins / chemistry*
Immunoprecipitation
Insulin-Like Growth Factor Binding Protein 4 / chemistry*
Mice
Mice, Inbred C3H
Myocytes, Cardiac / cytology*
Peptides / metabolism
Plasmids
Recombinant Proteins
Signal Transduction
Tissue Engineering
Wnt Proteins / antagonists & inhibitors
Wnt Proteins / genetics
Wnt Proteins / metabolism
beta Catenin / antagonists & inhibitors
beta Catenin / genetics
beta Catenin / metabolism
|
| IF |
3.978
|
| Times Cited |
8
|
|
WOS Category
|
CHEMISTRY, ANALYTICAL
|
| Resource |
| Human and Animal Cells |
P19CL6 |
