Reference - Detail
| RRC ID | 37611 |
|---|---|
| Author | Nishimura Y, Shimojima M, Tano Y, Miyamura T, Wakita T, Shimizu H. |
| Title | Human P-selectin glycoprotein ligand-1 is a functional receptor for enterovirus 71. |
| Journal | Nat Med |
| Abstract |
Enterovirus 71 (EV71) is a major causative agent of hand, foot and mouth disease (HFMD), a common febrile disease occurring mainly in young children. Although clinical manifestations of HFMD are usually mild and self limiting, a severe EV71 outbreak can lead to a diverse array of neurological diseases. Identification of the specific cellular receptors is crucial for elucidating the mechanism of early virus-host interactions and the pathogenesis of enteroviruses. Here we identify human P-selectin glycoprotein ligand-1 (PSGL-1; CD162), a sialomucin membrane protein expressed on leukocytes that has a major role in early stages of inflammation, as a functional receptor for EV71 using an expression cloning method by panning. The N-terminal region of PSGL-1 binds specifically to EV71. Stable PSGL-1 expression allowed EV71 entry and replication, and development of cytopathic effects in nonsusceptible mouse L929 cells. Five out of eight EV71 strains bound soluble PSGL-1 and used intact PSGL-1 as the primary receptor for infection of Jurkat T cells. Three other EV71 strains did not use PSGL-1, suggesting the presence of strain-specific replication of EV71 in leukocytes. EV71 replicated in nonleukocyte cell lines in a PSGL-1-independent manner, indicating the presence of alternative receptor(s) for EV71. The identification of PSGL-1 as a receptor for EV71 sheds new light on a role for PSGL-1-positive leukocytes in cell tropism and pathogenesis during the course of HFMD and other EV71-mediated diseases. |
| Volume | 15(7) |
| Pages | 794-7 |
| Published | 2009-7-1 |
| DOI | 10.1038/nm.1961 |
| PII | nm.1961 |
| PMID | 19543284 |
| MeSH | Animals Cell Line Enterovirus A, Human / pathogenicity* Enterovirus A, Human / physiology Humans Jurkat Cells Membrane Glycoproteins / analysis Membrane Glycoproteins / physiology* Mice Receptors, Virus / analysis Receptors, Virus / physiology* Virus Replication |
| IF | 36.13 |
| Times Cited | 258 |
| WOS Category | MEDICINE, RESEARCH & EXPERIMENTAL BIOCHEMISTRY & MOLECULAR BIOLOGY CELL BIOLOGY |
| Altmetric score |
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| The most frequently cited source | Patent(IFI CLAIMS) |
| Total number of mentions | 10 |
| Altmetric score changes over past 6months | 0.0 |
| Resource | |
| Human and Animal Cells | Jurkat(RCB0806) |