RRC ID 37617
著者 DeLyria ES, Redline RW, Blanchard TG.
タイトル Vaccination of mice against H pylori induces a strong Th-17 response and immunity that is neutrophil dependent.
ジャーナル Gastroenterology
Abstract BACKGROUND & AIMS:Vaccine efficacy against gastric Helicobacter pylori infection has been shown in mice, but little is known about the mechanisms of bacterial clearance. Our aim was to investigate a possible T-cell/neutrophil pathway of vaccine-induced protection.
METHODS:Nonimmune and immunized mice were compared for their response to H pylori challenge. T-cell responses were assessed by recall assays. Interleukin (IL)-17-induced chemokine production was evaluated by cytokine enzyme-linked immunosorbent assay. In a kinetic study, biopsy specimens were collected at multiple time points postchallenge and assessed for bacterial load and inflammation. Relative levels of T cells, IL-17, interferon gamma, MIP-2, KC, and LIX were determined by quantitative polymerase chain reaction. The role of neutrophils was evaluated by antibody-mediated depletion of neutrophils following challenge.
RESULTS:Immunization induced strong interferon gamma- and IL-17-producing T-cell responses, and IL-17 was capable of inducing significant amounts of KC and MIP-2 from dendritic cells, macrophages, fibroblasts, and gastric epithelial cells. Challenge of immunized mice induced significantly greater gastritis than that of infected mice, preceding significantly lower bacterial loads by day 7. In immune mice, T-cell recruitment to the gastric mucosa correlated with a continuous rise in IL-17 and interferon gamma levels, followed by KC, MIP-2, and LIX production and the recruitment of significant numbers of neutrophils by day 5. Antibody-mediated depletion of neutrophils abrogated vaccine efficacy.
CONCLUSIONS:Vaccination of mice against H pylori results in a significant Th-17 cell recall response associated with increases in chemokines that attract neutrophils to the stomach, which are important for eradication of H pylori.
巻・号 136(1)
ページ 247-56
公開日 2009-1-1
DOI 10.1053/j.gastro.2008.09.017
PII S0016-5085(08)01682-X
PMID 18948106
PMC PMC4960660
MeSH Animals Bacterial Vaccines / immunology* CD4-Positive T-Lymphocytes / immunology Cell Movement Chemokines / biosynthesis Female Gastric Mucosa / immunology Gastric Mucosa / microbiology Granulocyte Colony-Stimulating Factor / physiology Helicobacter pylori / immunology* Inflammation / etiology Interleukin-17 / biosynthesis* Mast Cells / physiology Mice Mice, Inbred C57BL Neutrophils / physiology* Vaccination
IF 17.373
引用数 122
WOS 分野 GASTROENTEROLOGY & HEPATOLOGY
リソース情報
ヒト・動物細胞 GSM06(RCB1779)