RRC ID 37626
Author Hasegawa S, Maruyama K, Takenaka H, Furukawa T, Saga T.
Title A medaka model of cancer allowing direct observation of transplanted tumor cells in vivo at a cellular-level resolution.
Journal Proc. Natl. Acad. Sci. U.S.A.
Abstract The recent success with small fish as an animal model of cancer with the aid of fluorescence technique has attracted cancer modelers' attention because it would be possible to directly visualize tumor cells in vivo in real time. Here, we report a medaka model capable of allowing the observation of various cell behaviors of transplanted tumor cells, such as cell proliferation and metastasis, which were visualized easily in vivo. We established medaka melanoma (MM) cells stably expressing GFP and transplanted them into nonirradiated and irradiated medaka. The tumor cells were grown at the injection sites in medaka, and the spatiotemporal changes were visualized under a fluorescence stereoscopic microscope at a cellular-level resolution, and even at a single-cell level. Tumor dormancy and metastasis were also observed. Interestingly, in irradiated medaka, accelerated tumor growth and metastasis of the transplanted tumor cells were directly visualized. Our medaka model provides an opportunity to visualize in vivo tumor cells "as seen in a culture dish" and would be useful for in vivo tumor cell biology.
Volume 106(33)
Pages 13832-7
Published 2009-8-18
DOI 10.1073/pnas.0903999106
PII 0903999106
PMID 19666513
PMC PMC2728980
MeSH Animals Cell Line, Tumor Cell Proliferation Cell Transplantation Disease Models, Animal* Green Fluorescent Proteins / metabolism Humans Melanoma, Amelanotic / metabolism Melanoma, Amelanotic / pathology Microscopy, Fluorescence / instrumentation* Microscopy, Fluorescence / methods Neoplasm Metastasis Neoplasm Transplantation / methods* Oryzias X-Rays
IF 9.504
Times Cited 6
Human and Animal Cells