| RRC ID |
37637
|
| Author |
Egusa H, Kaneda Y, Akashi Y, Hamada Y, Matsumoto T, Saeki M, Thakor DK, Tabata Y, Matsuura N, Yatani H.
|
| Title |
Enhanced bone regeneration via multimodal actions of synthetic peptide SVVYGLR on osteoprogenitors and osteoclasts.
|
| Journal |
Biomaterials
|
| Abstract |
Recently, the binding sequence Ser-Val-Val-Tyr-Gly-Leu-Arg (SVVYGLR) was found adjacent to the RGD sequence in osteopontin, suggesting involvement in osteo-immune cross-talk. The aim of this study was to investigate bioactive functions of a synthetic SVVYGLR peptide in osteoprogenitor cells and osteoclasts, and to examine potential applications in bone regeneration. The SVVYGLR peptide significantly enhanced the adhesion and proliferation of several human mesenchymal cells including bone marrow-derived mesenchymal stem cells, and alphavbeta3 integrin was involved in cell attachment to the peptide. Additionally, the peptide reduced the number of TRAP-positive multinucleated cells during osteoclastogenesis of RAW264.7 cells and normal murine pre-osteoclasts, and also suppressed NFAT activity and expression of osteoclastogenesis-related mRNAs. When standardized bone defects in rat calvariae were filled with a collagen sponge containing the peptide or PBS (control), the number of TRAP-positive osteoclasts in the grafted sites after 3 weeks was significantly lower in the peptide group. By the 5th week, significantly enhanced resorption of the grafted collagen sponge and new bone formation was observed within and surrounding the sponge in the peptide group. These data suggest that SVVYGLR is an effective bioactive peptide for bone tissue regeneration that promotes attachment and proliferation of osteogenic cells while also suppressing osteoclastogenesis.
|
| Volume |
30(27)
|
| Pages |
4676-86
|
| Published |
2009-9-1
|
| DOI |
10.1016/j.biomaterials.2009.05.032
|
| PII |
S0142-9612(09)00537-7
|
| PMID |
19520427
|
| MeSH |
Adult
Amino Acid Sequence
Animals
Bone Regeneration / drug effects*
Calcification, Physiologic / drug effects
Cell Adhesion / drug effects
Cell Line
Cell Proliferation / drug effects
Humans
Integrin alphaVbeta3
Male
Mesenchymal Stem Cells / cytology
Mesenchymal Stem Cells / drug effects
Mesenchymal Stem Cells / metabolism
Mice
Molecular Sequence Data
Osteoclasts / cytology
Osteoclasts / drug effects*
Osteoclasts / metabolism*
Osteogenesis / drug effects
Peptides / chemistry*
Peptides / pharmacology*
Rats
Rats, Sprague-Dawley
Skull / drug effects
Skull / pathology
Stem Cells / cytology
Stem Cells / drug effects*
Stem Cells / metabolism*
|
| IF |
10.317
|
| Times Cited |
43
|
|
WOS Category
|
ENGINEERING, BIOMEDICAL
MATERIALS SCIENCE, BIOMATERIALS
|
| Resource |
| Human and Animal Cells |
RAW 264(RCB0535) |
