論文 - 詳細
| RRC ID | 3765 |
|---|---|
| 著者 | Doan N, Gettins PG. |
| タイトル | alpha-Macroglobulins are present in some gram-negative bacteria: characterization of the alpha2-macroglobulin from Escherichia coli. |
| ジャーナル | J Biol Chem |
| Abstract |
alpha-Macroglobulins (alphaMs) are large glycoproteins that have been identified in a wide range of vertebrate and invertebrate species and are mostly thiol ester containing proteinase inhibitors. A recent analysis of bacterial genomes ( Budd, A., Blandin, S., Levashina, E. A., and Gibson, T. J. (2004) Genome Biol. 5, R38 ) identified many alpha-macroglobulin-like sequences that appear to have been acquired by Gram-negative bacteria from their metazoan hosts. We report the first expression and characterization of such a bacterial alpha-macroglobulin, that from Escherichia coli. This is also the first alpha-macroglobulin to be characterized that is predicted to be membrane-anchored. We found that the 183-kDa protein contains an intact thiol ester, is monomeric, and is localized to the periplasmic space. Reaction with proteinase results in limited cleavage within a bait region, rapid activation of the thiol ester, cross-linking to the attacking proteinase or other available nucleophiles, and partial protection of the proteinase against macromolecular substrates. Given these properties and the co-occurrence of the alphaM gene with one for a repair transglycosylase, this suggests a possible role for bacterial alphaMs in cell defense following host attack. Such a role would make bacterial alphaMs appropriate novel targets for antibiotic drugs. |
| 巻・号 | 283(42) |
| ページ | 28747-56 |
| 公開日 | 2008-10-17 |
| DOI | 10.1074/jbc.M803127200 |
| PII | S0021-9258(20)64271-5 |
| PMID | 18697741 |
| PMC | PMC2568910 |
| MeSH | Amino Acid Sequence Anti-Bacterial Agents / chemistry Cloning, Molecular Escherichia coli / metabolism* Esters / chemistry Gram-Negative Bacteria / metabolism* Humans Leukocyte Elastase / metabolism Models, Biological Molecular Sequence Data Spectrometry, Fluorescence / methods Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization Sulfhydryl Compounds / chemistry alpha-Macroglobulins / chemistry* alpha-Macroglobulins / metabolism* |
| IF | 4.238 |
| 引用数 | 17 |
| WOS 分野 | BIOCHEMISTRY & MOLECULAR BIOLOGY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 各媒体での言及数の合計 | 0 |
| リソース情報 | |
| 原核生物(大腸菌) | ME7986(MG1655) 2421(b2520) |
| 情報 | E. coli strain |