論文 - 詳細
| RRC ID | 37662 |
|---|---|
| 著者 | Okochi M, Takano S, Isaji Y, Senga T, Hamaguchi M, Honda H. |
| タイトル | Three-dimensional cell culture array using magnetic force-based cell patterning for analysis of invasive capacity of BALB/3T3/v-src. |
| ジャーナル | Lab Chip |
| Abstract |
A three-dimensional (3D) cell culture system has been fabricated using a magnetic force based cell patterning method, demonstrating a facile approach for the analysis of invasive capacity of BALB/3T3/v-src using an magnetic force and magnetite nanoparticles. The 3D cell patterning was performed using an external magnetic force and a pin holder, which enables the assembly of the magnetically labeled cells on the collagen gel-coated surface as array-like cell patterns, resulting in the development of a 3D in vitro culture model. The cells embedded in type I collagen showed a compacted, spheroid like configuration at each spot, and distinct, accelerated cell growth was observed in cancer model cells compared with the control cells. The developed 3D cell culture array was applied to the susceptibility assay of the GM6001 matrix metalloproteinase (MMP) inhibitor, a collagenase inhibitor; a distinct suppression of cell proliferation was observed, while little change was observed in 2D. The developed 3D cell culture array system is useful to assess the effects of pharmacologic and/or microenvironmental influences on tumor cell invasion. |
| 巻・号 | 9(23) |
| ページ | 3378-84 |
| 公開日 | 2009-12-7 |
| DOI | 10.1039/b909304d |
| PMID | 19904404 |
| MeSH | Animals Anticarcinogenic Agents / pharmacology BALB 3T3 Cells Cell Culture Techniques / instrumentation* Cell Line, Transformed Cell Proliferation / drug effects Collagen Type I / chemistry Dipeptides / pharmacology Equipment Design Genes, src Genistein / pharmacology Liposomes / chemistry Liposomes / metabolism Magnetics* Magnetite Nanoparticles / chemistry Matrix Metalloproteinase Inhibitors Mice Neoplasm Invasiveness / diagnosis* Protease Inhibitors / pharmacology |
| IF | 6.774 |
| 引用数 | 50 |
| WOS 分野 | BIOCHEMICAL RESEARCH METHODS CHEMISTRY, MULTIDISCIPLINARY NANOSCIENCE & NANOTECHNOLOGY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | Patent(IFI CLAIMS) |
| 各媒体での言及数の合計 | 3 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| ヒト・動物細胞 | |