| RRC ID |
37663
|
| 著者 |
Mavis B, Demirtaş TT, Gümüşderelioğlu M, Gündüz G, Colak U.
|
| タイトル |
Synthesis, characterization and osteoblastic activity of polycaprolactone nanofibers coated with biomimetic calcium phosphate.
|
| ジャーナル |
Acta Biomater
|
| Abstract |
Immersion of electrospun polycaprolactone (PCL) nanofiber mats in calcium phosphate solutions similar to simulated body fluid resulted in deposition of biomimetic calcium phosphate layer on the nanofibers and thus a highly bioactive novel scaffold has been developed for bone tissue engineering. Coatings with adequate integrity, favorable chemistry and morphology were achieved in less than 6h of immersion. In the coating solutions, use of lower concentrations of phosphate sources with respect to the literature values (i.e., 3.62 vs. 10 mM) was substantiated by a thermodynamic modeling approach. Recipe concentration combinations that were away from the calculated dicalcium phosphate phase stability region resulted in micron-sized calcium phosphates with native nanostructures. While the nano/microstructure formed by the deposited calcium phosphate layer is controlled by increasing the solution pH to above 6.5 and increasing the duration of immersion experimentally, the nanostructure imposed by the dimensions of the fibers was controlled by the polymer concentration (12% w/v), applied voltage (25 kV) and capillary tip to collector distance (35 cm). The deposited coating increased quantitatively by extending the soak up to 6h. On the other hand, the porosity values attained in the scaffolds were around 87% and the biomimetic coatings did not alter the nanofiber mat porosities negatively since the deposition continued along the fibers after the first 2h. Upon confirming the non-toxic nature of the electrospun PCL nanofiber mats, the effects of different nano/microstructures formed were evaluated by the osteoblastic activity. The levels of both alkaline phosphatase activity and osteocalcin were found to be higher in the coated PCL nanofibers than in the uncoated PCL nanofibers, indicating that biomimetic calcium phosphate on PCL nanofibers supports osteoblastic differentiation.
|
| 巻・号 |
5(8)
|
| ページ |
3098-111
|
| 公開日 |
2009-10-1
|
| DOI |
10.1016/j.actbio.2009.04.037
|
| PII |
S1742-7061(09)00201-3
|
| PMID |
19426840
|
| MeSH |
3T3 Cells
Absorption
Animals
Biomimetic Materials / chemical synthesis*
Bone Substitutes / chemistry*
Calcium Phosphates / chemistry*
Cell Proliferation
Crystallization / methods
Extracellular Matrix / chemistry
Materials Testing
Mice
Nanostructures / ultrastructure
Osteoblasts / cytology*
Osteoblasts / physiology*
Osteogenesis / physiology*
Particle Size
Polyesters / chemistry*
Porosity
Surface Properties
Tissue Engineering / methods*
|
| IF |
7.242
|
| 引用数 |
106
|
|
WOS 分野
|
ENGINEERING, BIOMEDICAL
MATERIALS SCIENCE, BIOMATERIALS
|
| リソース情報 |
| ヒト・動物細胞 |
MC3T3-E1(RCB1126) |
