| RRC ID |
37665
|
| Author |
Wang X, Ito A, Sogo Y, Li X, Tsurushima H, Oyane A.
|
| Title |
Ascorbate-apatite composite and ascorbate-FGF-2-apatite composite layers formed on external fixation rods and their effects on cell activity in vitro.
|
| Journal |
Acta Biomater
|
| Abstract |
Ascorbate-apatite and ascorbate-fibroblast growth factor-2 (FGF-2)-apatite composite layers were successfully formed on anodically oxidized Ti rods clinically used for external fixation by a one-step procedure at 25 degrees C, using a metastable supersaturated calcium phosphate solution supplemented with l-ascorbic acid phosphate magnesium salt n-hydrate (AsMg) and FGF-2. The AsMg-apatite and AsMg-FGF-2-apatite composite layers were evaluated in vitro using fibroblastic NIH3T3 and osteoblastic MC3T3-E1 cells. The AsMg-FGF-2-apatite composite layer markedly enhanced the NIH3T3 cell proliferation and procollagen type capital I, Ukrainian gene expression. Without FGF-2, the AsMg-apatite composite layer whose ascorbate content was 3.64+/-1.27microgcm(-2) obviously enhanced osteoblastic proliferation and differentiation. However, the AsMg-FGF-2-apatite composite layers whose FGF-2 contents were from 0.15+/-0.03 to 0.31+/-0.04microgcm(-2) inhibited osteoblastic differentiation in vitro. Thus, the AsMg-FGF-2-apatite composite layer should be precipitated on the surface of external fixators attached to skin and soft tissue. On the other hand, the AsMg-apatite composite layer should be precipitated at the part attached to bone tissue.
|
| Volume |
5(7)
|
| Pages |
2647-56
|
| Published |
2009-9-1
|
| DOI |
10.1016/j.actbio.2009.03.020
|
| PII |
S1742-7061(09)00121-4
|
| PMID |
19375998
|
| MeSH |
Animals
Apatites / chemistry*
Ascorbic Acid / chemistry*
Cell Proliferation / drug effects*
Cell Survival / drug effects*
Coated Materials, Biocompatible / chemistry
Coated Materials, Biocompatible / pharmacology*
External Fixators*
Fibroblast Growth Factor 2 / chemistry
Fibroblast Growth Factor 2 / pharmacology*
Mice
NIH 3T3 Cells
|
| IF |
7.242
|
| Times Cited |
23
|
|
WOS Category
|
ENGINEERING, BIOMEDICAL
MATERIALS SCIENCE, BIOMATERIALS
|
| Resource |
| Human and Animal Cells |
MC3T3-E1(RCB1126) |
