論文 - 詳細
| RRC ID | 37685 |
|---|---|
| 著者 | Ueno K, Hazama S, Mitomori S, Nishioka M, Suehiro Y, Hirata H, Oka M, Imai K, Dahiya R, Hinoda Y. |
| タイトル | Down-regulation of frizzled-7 expression decreases survival, invasion and metastatic capabilities of colon cancer cells. |
| ジャーナル | Br J Cancer |
| Abstract |
BACKGROUND:The canonical Wnt signalling pathway is activated in most sporadic colorectal cancers (CRCs). We previously reported that FZD7 functions as a receptor for the canonical Wnt signalling pathway in colon cancer cells. METHODS AND RESULTS:In this study, we examined the function of FZD7 in survival, invasion and metastatic capabilities of colon cancer cells. FZD7_siRNA transfection decreased cell viability of HT-29 and HCT-116 colon cancer cells. Expression of c-Jun, phosphorylation of JNK and c-Jun, and activation of RhoA were suppressed after FZD7_siRNA transfection into HCT-116 cells. In vitro invasion activity and Wnt target gene expression were also reduced in HCT-116 cells transfected with FZD7_siRNA. Liver metastasis of stable FZD7_siRNA HCT-116 cell transfectants in scid mice was decreased to 40-50% compared to controls. The mRNA levels of FZD7 in 135 primary CRC tissues were examined by real-time PCR. FZD7 mRNA levels were significantly higher in stage II, III or IV tumours than in non-tumour tissues (P<0.005), and overall survival was shorter in those patients with higher FZD7 expression (P<0.001). CONCLUSION:These data suggest that FZD7 may be involved in enhancement of survival, invasion and metastatic capabilities of colon cancer cells through non-canonical Wnt signalling pathways as well as the canonical pathway. |
| 巻・号 | 101(8) |
| ページ | 1374-81 |
| 公開日 | 2009-10-20 |
| DOI | 10.1038/sj.bjc.6605307 |
| PII | 6605307 |
| PMID | 19773752 |
| PMC | PMC2768449 |
| MeSH | Animals Cell Survival Colorectal Neoplasms / pathology* Frizzled Receptors / antagonists & inhibitors Frizzled Receptors / genetics Frizzled Receptors / physiology* HCT116 Cells HT29 Cells Humans Liver Neoplasms, Experimental / secondary Mice Mice, SCID Neoplasm Invasiveness RNA, Messenger / analysis RNA, Small Interfering / genetics Receptors, G-Protein-Coupled / antagonists & inhibitors Receptors, G-Protein-Coupled / genetics Receptors, G-Protein-Coupled / physiology* |
| IF | 5.791 |
| 引用数 | 77 |
| WOS 分野 | ONCOLOGY |
| リソース情報 | |
| ヒト・動物細胞 | 293T(RCB2202) |