| RRC ID |
37696
|
| 著者 |
Niki T, Tsutsui S, Hirose S, Aradono S, Sugimoto Y, Takeshita K, Nishi N, Hirashima M.
|
| タイトル |
Galectin-9 is a high affinity IgE-binding lectin with anti-allergic effect by blocking IgE-antigen complex formation.
|
| ジャーナル |
J Biol Chem
|
| Abstract |
Galectin (Gal)-9 was first described as an eosinophil chemoattractant. With the progress in research, Gal-9 has come to be known as a versatile immunomodulator that is involved in various aspects of immune regulations, and the entire picture of the function still remains elusive. To uncover as-yet unknown activity of Gal-9, we have been examining the effect of the protein in various disease animal models. Here we show that Gal-9 attenuated asthmatic reaction in guinea pigs and suppressed passive-cutaneous anaphylaxis in mice. These results indicate the mast cell stabilizing effect of Gal-9. In vitro studies of mast cell degranulation involving RBL-2H3 cells demonstrated that Gal-9 suppressed degranulation from the cells stimulated by IgE plus antigen and that the inhibitory effect was completely abrogated in the presence of lactose, indicating lectin activity of Gal-9 is critical. We found that Gal-9 strongly and specifically bound IgE, which is a heavily glycosylated immunoglobulin, and that the interaction prevented IgE-antigen complex formation, clarifying the mode of action of the anti-degranulation effect. Gal-9 is expressed by several mast cells including mouse mast cell line MC/9. The fact that immunological stimuli of MC/9 cells augmented Gal-9 secretion from the cells implies that Gal-9 is an autocrine regulator of mast cell function to suppress excessive degranulation. Collectively, these findings shed light on a novel function of Gal-9 in mast cells and suggest a beneficial utility of Gal-9 for the treatment of allergic disorders including asthma.
|
| 巻・号 |
284(47)
|
| ページ |
32344-52
|
| 公開日 |
2009-11-20
|
| DOI |
10.1074/jbc.M109.035196
|
| PII |
S0021-9258(20)37826-1
|
| PMID |
19776007
|
| PMC |
PMC2781649
|
| MeSH |
Animals
Antigens / chemistry*
Cell Line, Tumor
Female
Galectins / metabolism*
Guinea Pigs
Humans
Immunoglobulin E / chemistry*
Jurkat Cells
Lectins / chemistry*
Male
Mice
Mice, Inbred BALB C
Protein Binding
Rats
Receptors, IgE / chemistry*
|
| IF |
4.238
|
| 引用数 |
87
|
|
WOS 分野
|
BIOCHEMISTRY & MOLECULAR BIOLOGY
|
| リソース情報 |
| ヒト・動物細胞 |
RBL 2H3(RCB2782) |
