Reference - Detail
| RRC ID | 37699 |
|---|---|
| Author | Kanoh N, Takayama H, Honda K, Moriya T, Teruya T, Simizu S, Osada H, Iwabuchi Y. |
| Title | Cleavable linker for photo-cross-linked small-molecule affinity matrix. |
| Journal | Bioconjug Chem |
| Abstract |
The introduction of a cleavable site in a photoactivatable linker, which is used to immobilize small molecules on an affinity matrix via a site-nonselective carbene addition/insertion reaction, makes it possible to verify the presence of the immobilized small molecule on the affinity matrix. It also permits the efficient detection of proteins covalently bound to the immobilized small molecule. |
| Volume | 21(1) |
| Pages | 182-6 |
| Published | 2010-1-1 |
| DOI | 10.1021/bc900316q |
| PMID | 20028022 |
| MeSH | Binding Sites Blotting, Western Chromatography, Liquid Cross-Linking Reagents / chemistry* Disulfides / chemistry Enzymes, Immobilized / chemistry Enzymes, Immobilized / metabolism Ligands Mass Spectrometry Methane / analogs & derivatives* Methane / chemistry Microspheres Organophosphorus Compounds / chemistry Photoaffinity Labels / chemistry* Photochemistry* Protein Binding Protein Phosphatase 2 / chemistry Protein Phosphatase 2 / metabolism Sepharose / chemistry |
| IF | 4.031 |
| Times Cited | 20 |
| WOS Category | BIOCHEMICAL RESEARCH METHODS CHEMISTRY, ORGANIC CHEMISTRY, MULTIDISCIPLINARY BIOCHEMISTRY & MOLECULAR BIOLOGY |
| Resource | |
| Human and Animal Cells | |