| RRC ID |
37709
|
| 著者 |
Aoki N, Kimura Y, Kimura S, Nagato T, Azumi M, Kobayashi H, Sato K, Tateno M.
|
| タイトル |
Expression and functional role of MDL-1 (CLEC5A) in mouse myeloid lineage cells.
|
| ジャーナル |
J Leukoc Biol
|
| Abstract |
Myeloid DNAX activation protein 12 (DAP12)-associating lectin-1 (MDL-1), also known as C-type lectin domain family 5, member A, is a type II transmembrane protein belonging to the C-type lectin family and associates with DAP12 (also called KARAP or TYROBP). It has been reported that two isoforms of MDL-1-long form (MDL-1L) and short form (MDL-1S)-exist in mice. Previously, we observed the marked induction of MDL-1 mRNA expression during the pulmonary mycobacterial infection in mice. The data suggested that the MDL-1-expressing cells were involved in immune responses against mycobacterial infection; however, little is known about the function of MDL-1 as yet. In this study, we demonstrated the significant protein expression of MDL-1L and MDL-1S in mouse neutrophils and macrophages. MDL-1L was highly glycosylated by N-linked glycan and sialic acid. Interestingly, the expression pattern of MDL-1 was different between neutrophils and macrophages. MDL-1 expression was notably induced during the differentiation of the mouse myeloid cell line 32Dcl3 into neutrophils. Additionally, we observed that MDL-1 stimulation induced a significant amount of RANTES and macrophage-derived chemokine production in 32Dcl3 cells in cooperation with signaling through TLR. MDL-1 stimulation also up-regulated CD11b expression and maintained cell survival. Our findings indicate that MDL-1, therefore, plays an important role in immune defense as a result of an innate immunity, which involves neutrophils and macrophages.
|
| 巻・号 |
85(3)
|
| ページ |
508-17
|
| 公開日 |
2009-3-1
|
| DOI |
10.1189/jlb.0508329
|
| PII |
jlb.0508329
|
| PMID |
19074552
|
| MeSH |
Animals
Cell Differentiation
Cell Line
Cell Survival
Chemokines / biosynthesis
Glycosylation
Immunity, Innate*
Lectins, C-Type / genetics
Lectins, C-Type / physiology*
Macrophages / metabolism*
Mice
Myeloid Cells / cytology*
Neutrophils / metabolism*
Protein Isoforms
Receptors, Cell Surface / genetics
Receptors, Cell Surface / physiology*
|
| IF |
3.757
|
| 引用数 |
20
|
|
WOS 分野
|
IMMUNOLOGY
HEMATOLOGY
CELL BIOLOGY
|
| リソース情報 |
| ヒト・動物細胞 |
32Dcl3(RCB1538) |
