論文 - 詳細
| RRC ID | 37714 |
|---|---|
| 著者 | Fujita H, Shimizu K, Nagamori E. |
| タイトル | Application of a cell sheet-polymer film complex with temperature sensitivity for increased mechanical strength and cell alignment capability. |
| ジャーナル | Biotechnol Bioeng |
| Abstract |
We have succeeded in fabricating a cell sheet-polymer film complex involving a temperature-sensitive polymer that has enough mechanical strength that can be manipulated even by forceps. The polymer film can be removed by lowering the temperature after transplantation, demonstrating its potential use in regenerative medicine. Recently, tissue engineering involving cell sheets was developed, tissues being fabricated by layering of these cell sheets. This technique promises high density cell packing, which is important for native cell functions, and successful heart therapy using cardiac cell sheets has been reported. On the other hand, the fabrication of a large tissue using cell sheets is difficult because of fragility of the cell sheets. Here, we have developed a novel method in which cells are attached to a temperature-sensitive poly-N-isopropylacrylamide film mixed with laminin and collagen IV, and report that the cell sheet-polymer film complex can be manipulated with forceps. A cell sheet can be removed from the polymer film by lowering the temperature after the manipulation. We have utilized this technique for the primary myocardium and fabricated a physiologically active multi-layered cardiac cell sheet. By applying a micropattern to this polymer film, we have succeeded in making a skeletal muscle cell sheet in which myotubes are oriented in the desired direction. Overall, we showed that this method is useful for cell sheet manipulation, morphogenesis, and transplantation. |
| 巻・号 | 103(2) |
| ページ | 370-7 |
| 公開日 | 2009-6-1 |
| DOI | 10.1002/bit.22251 |
| PMID | 19205047 |
| MeSH | Acrylic Resins* Amino Acids, Diamino Cell Proliferation* Collagen Myocardium Regenerative Medicine / methods Temperature* Tissue Engineering / methods* |
| IF | 4.002 |
| 引用数 | 28 |
| WOS 分野 | BIOTECHNOLOGY & APPLIED MICROBIOLOGY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | Patent(IFI CLAIMS) |
| 各媒体での言及数の合計 | 5 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| ヒト・動物細胞 | NIH3T3 10T1/2(RCB0247) C2C12(RCB0987) |