Reference - Detail
RRC ID | 37753 |
---|---|
Author | Kawahara Y, Manabe T, Matsumoto M, Kajiume T, Matsumoto M, Yuge L. |
Title | LIF-free embryonic stem cell culture in simulated microgravity. |
Journal | PLoS One |
Abstract |
BACKGROUND:Leukemia inhibitory factor (LIF) is an indispensable factor for maintaining mouse embryonic stem (ES) cell pluripotency. A feeder layer and serum are also needed to maintain an undifferentiated state, however, such animal derived materials need to be eliminated for clinical applications. Therefore, a more reliable ES cell culture technique is required. METHODOLOGY/PRINCIPAL FINDINGS:We cultured mouse ES cells in simulated microgravity using a 3D-clinostat. We used feeder-free and serum-free media without LIF. CONCLUSIONS/SIGNIFICANCE:Here we show that simulated microgravity allows novel LIF-free and animal derived material-free culture methods for mouse ES cells. |
Volume | 4(7) |
Pages | e6343 |
Published | 2009-7-23 |
DOI | 10.1371/journal.pone.0006343 |
PMID | 19626124 |
PMC | PMC2710515 |
MeSH | Animals Base Sequence Cell Culture Techniques DNA Primers Embryonic Stem Cells / cytology* Leukemia Inhibitory Factor / metabolism* Mice Polymerase Chain Reaction Weightlessness* |
IF | 2.74 |
Times Cited | 30 |
WOS Category | CELL BIOLOGY |
Resource | |
Human and Animal Cells | BRC6(AES0010) |