論文 - 詳細
| RRC ID | 37754 |
|---|---|
| 著者 | Harada Y, Ueda Y, Kinoh H, Komaru A, Fuji-Ogawa T, Furuya A, Iida A, Hasegawa M, Ichikawa T, Yonemitsu Y. |
| タイトル | Cytokine-based log-scale expansion of functional murine dendritic cells. |
| ジャーナル | PLoS One |
| Abstract |
BACKGROUND:Limitations of the clinical efficacy of dendritic cell (DC)-based immunotherapy, as well as difficulties in their industrial production, are largely related to the limited number of autologous DCs from each patient. We here established a possible breakthrough, a simple and cytokine-based culture method to realize a log-scale order of functional murine DCs (>1,000-fold), which cells were used as a model before moving to human studies. METHODOLOGY/PRINCIPAL FINDINGS:Floating cultivation of lineage-negative hematopoietic progenitors from bone marrow in an optimized cytokine cocktail (FLT3-L, IL-3, IL-6, and SCF) led to a stable log-scale proliferation of these cells, and a subsequent differentiation study using IL-4/GM-CSF revealed that 3-weeks of expansion was optimal to produce CD11b+/CD11c+ DC-like cells. The expanded DCs had typical features of conventional myeloid DCs in vitro and in vivo, including identical efficacy as tumor vaccines. CONCLUSIONS/SIGNIFICANCE:The concept of DC expansion should make a significant contribution to the progress of DC-based immunotherapy. |
| 巻・号 | 4(8) |
| ページ | e6674 |
| 公開日 | 2009-8-18 |
| DOI | 10.1371/journal.pone.0006674 |
| PMID | 19688095 |
| PMC | PMC2723913 |
| MeSH | Animals Cytokines / physiology* Dendritic Cells / cytology* Dendritic Cells / immunology Enzyme-Linked Immunosorbent Assay Female Lymphocyte Culture Test, Mixed Male Mice Mice, Inbred BALB C Mice, Inbred C3H Mice, Inbred C57BL |
| IF | 2.74 |
| 引用数 | 7 |
| WOS 分野 | IMMUNOLOGY |
| リソース情報 | |
| ヒト・動物細胞 | LM8(RCB1450) |