| RRC ID |
37758
|
| 著者 |
Osabe M, Sugatani J, Takemura A, Kurosawa M, Yamazaki Y, Ikari A, Miwa M.
|
| タイトル |
Up-regulation of CAR expression through Elk-1 in HepG2 and SW480 cells by serum starvation stress.
|
| ジャーナル |
FEBS Lett
|
| Abstract |
Constitutive androstane receptor (CAR) is a transcription factor regulating the expression of several genes related to drug metabolism. CAR expression was elevated in human HepG2 and SW480 cells by serum starvation. From reporter gene assays, mutagenesis, RNA interference, and chromatin immunoprecipitation assays, we identified the serum response element at -142/-139 in the CAR gene transactivated by Elk-1. Whereas treatment with U0126 (ERK inhibitor) enhanced CAR expression, SP600125 (stress-activated protein kinase inhibitor, SAPK) suppressed the phosphorylation of Elk-1 caused by serum-starvation stress and the elevation of CAR mRNA, suggesting that CAR expression may be mediated by phosphorylated Elk-1 via the SAPK signaling pathway.
|
| 巻・号 |
583(5)
|
| ページ |
885-9
|
| 公開日 |
2009-3-4
|
| DOI |
10.1016/j.febslet.2009.01.051
|
| PII |
S0014-5793(09)00093-3
|
| PMID |
19302787
|
| MeSH |
5' Flanking Region / genetics
Anthracenes / pharmacology
Base Sequence
Cell Line, Tumor
Constitutive Androstane Receptor
Culture Media, Serum-Free
Humans
Molecular Sequence Data
Phosphorylation / drug effects
Protein Binding
RNA, Messenger / genetics
Receptors, Cytoplasmic and Nuclear / genetics
Receptors, Cytoplasmic and Nuclear / metabolism*
Signal Transduction
Stress, Physiological*
Transcription Factors / genetics
Transcription Factors / metabolism*
Transcriptional Activation / genetics
Up-Regulation*
ets-Domain Protein Elk-1 / genetics
ets-Domain Protein Elk-1 / metabolism*
|
| IF |
3.057
|
| 引用数 |
10
|
|
WOS 分野
|
BIOPHYSICS
BIOCHEMISTRY & MOLECULAR BIOLOGY
CELL BIOLOGY
|
| リソース情報 |
| ヒト・動物細胞 |
|
