| RRC ID |
37802
|
| 著者 |
Tsuji M, Yamamoto H, Sato T, Mizuha Y, Kawai Y, Taketani Y, Kato S, Terao J, Inakuma T, Takeda E.
|
| タイトル |
Dietary quercetin inhibits bone loss without effect on the uterus in ovariectomized mice.
|
| ジャーナル |
J Bone Miner Metab
|
| Abstract |
Quercetin is a major dietary flavonoid found in onions and other vegetables, and potentially has beneficial effects on disease prevention. In the present study, we demonstrate for the first time the effects of dietary quercetin on bone loss and uterine weight loss by ovariectomy in vivo. Female mice were ovariectomized (OVX) and were randomly allocated to 3 groups: a control diet or a diet with 0.25% (LQ) or 2.5% quercetin (HQ). After 4 weeks, dietary quercetin had no effects on uterine weight in OVX mice, but bone mineral density of the lumbar spine L4 and femur measured by peripheral quantitative computed tomography (pQCT) was higher in both the sham and the HQ groups than in the OVX group. Histomorphometric analysis showed that the HQ group restored bone volume (BV/TV) completely in distal femoral cancellous bone, but did not reduce the osteoclast surface area and osteoclast number when compared with the OVX group. In in-vitro experiments using mouse monocyte/macrophage cell line RAW264.7 cells, however, quercetin and its conjugate, quercetin-3-O-beta-D: -glucuronide dose-dependently inhibited the receptor activator of nuclear factor-kappa B ligand (RANKL)-induced osteoclast differentiation, and the RANKL-stimulated expression of osteoclast related genes was also inhibited by quercetin. The luciferase reporter assay showed that quercetin did not appear to have estrogenic activity through estrogen receptors. These results suggest that dietary quercetin inhibits bone loss without effect on the uterus in OVX mice and does not act as a potent inhibitor of osteoclastogenesis or as a selective estrogen receptor modulator in vivo.
|
| 巻・号 |
27(6)
|
| ページ |
673-81
|
| 公開日 |
2009-1-1
|
| DOI |
10.1007/s00774-009-0088-0
|
| PMID |
19495926
|
| MeSH |
Animals
Body Weight
Bone Density
Bone and Bones / metabolism
Cell Line, Tumor
Dose-Response Relationship, Drug
Female
Flavonoids / metabolism
HeLa Cells
Humans
Lumbar Vertebrae / metabolism
Mice
Mice, Inbred C57BL
NIH 3T3 Cells
Ovariectomy / methods*
Quercetin / analogs & derivatives
Quercetin / pharmacology*
RANK Ligand / metabolism
Time Factors
Tomography, X-Ray Computed / methods
Uterus / drug effects*
|
| IF |
2.297
|
| 引用数 |
61
|
|
WOS 分野
|
MEDICINE, RESEARCH & EXPERIMENTAL
ENDOCRINOLOGY & METABOLISM
|
| リソース情報 |
| ヒト・動物細胞 |
HeLa(RCB0007)
RAW 264(RCB0535)
NIH3T3
MCF7(RCB1904) |
