論文 - 詳細
| RRC ID | 37811 |
|---|---|
| 著者 | Nagamine T, Hayakawa K, Kusakabe T, Takada H, Nakazato K, Hisanaga E, Iha M. |
| タイトル | Inhibitory effect of fucoidan on Huh7 hepatoma cells through downregulation of CXCL12. |
| ジャーナル | Nutr Cancer |
| Abstract |
The aim of this study is to assess whether fucoidan modulates the expression of chemokine ligand 12 (CXCL12)/chemokine receptor 4 (CXCR4) and exerts antitumor activity toward Huh7 hepatoma cells. According to 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assays, fucoidan inhibited the growth of Huh7 cells and HepG2 cells in a dose-dependent manner, with a 50% inhibition of cell growth (IC50) of 2.0 and 4.0 mg/ml, respectively. alpha-fetoprotein levels in medium collected from fucoidan-treated cells were significantly decreased in Huh7 cells but not in HepG2 cells. Western blotting revealed that the amount of alpha-fetoprotein was decreased by 1.0 mg/ml of fucoidan in Huh7 cells, whereas it was unchanged in HepG2 cells. In Huh7 cells, CXCL12 mRNA expression was significantly downregulated by 1.0 mg/ml of fucoidan, whereas CXCR4 mRNA expression was unchanged by fucoidan. CXCL12 and CXCR4 mRNA were barely expressed in HepG2 cells. In addition, 1.0 mg/ml of fucoidan mildly arrested the cell cycle and induced apoptosis in Huh7 cells. The findings suggest that fucoidan exhibits antitumor activity toward Huh7 cells through the downregulation of CXCL12 expression. |
| 巻・号 | 61(3) |
| ページ | 340-7 |
| 公開日 | 2009-1-1 |
| DOI | 10.1080/01635580802567133 |
| PII | 910506533 |
| PMID | 19373607 |
| MeSH | Antineoplastic Agents / pharmacology* Apoptosis / drug effects Blotting, Western Carcinoma, Hepatocellular / drug therapy* Carcinoma, Hepatocellular / pathology Cell Cycle / drug effects Cell Line, Tumor Cell Survival / drug effects Chemokine CXCL12 / analysis Chemokine CXCL12 / antagonists & inhibitors* Enzyme-Linked Immunosorbent Assay Humans In Situ Nick-End Labeling Liver Neoplasms / drug therapy* Liver Neoplasms / pathology Polymerase Chain Reaction Polysaccharides / pharmacology* alpha-Fetoproteins / analysis |
| IF | 2.363 |
| 引用数 | 53 |
| WOS 分野 | NUTRITION & DIETETICS ONCOLOGY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | Patent(IFI CLAIMS) |
| 各媒体での言及数の合計 | 3 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| ヒト・動物細胞 | HuH-7(RCB1366) Hep G2 |