| RRC ID |
37811
|
| 著者 |
Nagamine T, Hayakawa K, Kusakabe T, Takada H, Nakazato K, Hisanaga E, Iha M.
|
| タイトル |
Inhibitory effect of fucoidan on Huh7 hepatoma cells through downregulation of CXCL12.
|
| ジャーナル |
Nutr Cancer
|
| Abstract |
The aim of this study is to assess whether fucoidan modulates the expression of chemokine ligand 12 (CXCL12)/chemokine receptor 4 (CXCR4) and exerts antitumor activity toward Huh7 hepatoma cells. According to 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assays, fucoidan inhibited the growth of Huh7 cells and HepG2 cells in a dose-dependent manner, with a 50% inhibition of cell growth (IC50) of 2.0 and 4.0 mg/ml, respectively. alpha-fetoprotein levels in medium collected from fucoidan-treated cells were significantly decreased in Huh7 cells but not in HepG2 cells. Western blotting revealed that the amount of alpha-fetoprotein was decreased by 1.0 mg/ml of fucoidan in Huh7 cells, whereas it was unchanged in HepG2 cells. In Huh7 cells, CXCL12 mRNA expression was significantly downregulated by 1.0 mg/ml of fucoidan, whereas CXCR4 mRNA expression was unchanged by fucoidan. CXCL12 and CXCR4 mRNA were barely expressed in HepG2 cells. In addition, 1.0 mg/ml of fucoidan mildly arrested the cell cycle and induced apoptosis in Huh7 cells. The findings suggest that fucoidan exhibits antitumor activity toward Huh7 cells through the downregulation of CXCL12 expression.
|
| 巻・号 |
61(3)
|
| ページ |
340-7
|
| 公開日 |
2009-1-1
|
| DOI |
10.1080/01635580802567133
|
| PII |
910506533
|
| PMID |
19373607
|
| MeSH |
Antineoplastic Agents / pharmacology*
Apoptosis / drug effects
Blotting, Western
Carcinoma, Hepatocellular / drug therapy*
Carcinoma, Hepatocellular / pathology
Cell Cycle / drug effects
Cell Line, Tumor
Cell Survival / drug effects
Chemokine CXCL12 / analysis
Chemokine CXCL12 / antagonists & inhibitors*
Enzyme-Linked Immunosorbent Assay
Humans
In Situ Nick-End Labeling
Liver Neoplasms / drug therapy*
Liver Neoplasms / pathology
Polymerase Chain Reaction
Polysaccharides / pharmacology*
alpha-Fetoproteins / analysis
|
| IF |
2.363
|
| 引用数 |
53
|
|
WOS 分野
|
NUTRITION & DIETETICS
ONCOLOGY
|
| リソース情報 |
| ヒト・動物細胞 |
HuH-7(RCB1366)
Hep G2 |
