RRC ID |
37815
|
Author |
Hisatsune A, Kawasaki M, Nakayama H, Mikami Y, Miyata T, Isohama Y, Katsuki H, Kim KC.
|
Title |
Internalization of MUC1 by anti-MUC1 antibody from cell membrane through the macropinocytotic pathway.
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Journal |
Biochem Biophys Res Commun
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Abstract |
MUC1 is a type I transmembrane glycoprotein aberrantly overexpressed in various cancer cells. It is thought to serve as a physical barrier from the extracellular environment and also as a receptor for various extracellular molecules. However, little is known about the fate of MUC1 during and after the interaction with these molecules. In the present study, we used anti-MUC1 antibody as an interacting molecule and investigated the cellular trafficking of MUC1. Our results showed that: (1) anti-MUC1 antibody was internalized only in MUC1 expressing cells and triggered internalization and down-regulation of MUC1; (2) the internalization of MUC1 by anti-MUC1 antibody required the cytoplasmic tail of MUC1 and was suppressed by inhibitors of Na(+)/H(+) exchanger, and caveola/raft-dependent internalization, but not by an inhibitor of clathrin-dependent internalization. We conclude that antibody-induced internalization of MUC1 involves the macropinocytotic pathway.
|
Volume |
388(4)
|
Pages |
677-82
|
Published |
2009-10-30
|
DOI |
10.1016/j.bbrc.2009.08.059
|
PII |
S0006-291X(09)01628-3
|
PMID |
19683510
|
MeSH |
Antibodies, Monoclonal / immunology*
Cell Line
Cell Membrane / metabolism*
Humans
Mucin-1 / genetics
Mucin-1 / immunology
Mucin-1 / metabolism*
Pinocytosis*
Protein Transport
|
IF |
2.985
|
Times Cited |
7
|
WOS Category
|
BIOPHYSICS
BIOCHEMISTRY & MOLECULAR BIOLOGY
|
Resource |
Human and Animal Cells |
|