RRC ID 3782
Author Nagata M, Murakami E, Shimoda Y, Shimoda-Sasakura F, Kucho K, Suzuki A, Abe M, Higashi S, Uchiumi T.
Title Expression of a class 1 hemoglobin gene and production of nitric oxide in response to symbiotic and pathogenic bacteria in Lotus japonicus.
Journal Mol. Plant Microbe Interact.
Abstract Symbiotic nitrogen fixation by the collaboration between leguminous plants and rhizobia is an important system in the global nitrogen cycle, and some molecular aspects during the early stage of host-symbiont recognition have been revealed. To understand the responses of a host plant against various bacteria, we examined expression of hemoglobin (Hb) genes and production of nitric oxide (NO) in Lotus japonicus after inoculation with rhizobia or plant pathogens. When the symbiotic rhizobium Mesorhizobium loti was inoculated, expression of LjHb1 and NO production were induced transiently in the roots at 4 h after inoculation. In contrast, inoculation with the nonsymbiotic rhizobia Sinorhizobium meliloti and Bradyrhizobium japonicum induced neither expression of LjHb1 nor NO production. When L. japonicus was inoculated with plant pathogens (Ralstonia solanacearum or Pseudomonas syringae), continuous NO production was observed in roots but induction of LjHb1 did not occur. These results suggest that modulation of NO levels and expression of class 1 Hb are involved in the establishment of the symbiosis.
Volume 21(9)
Pages 1175-83
Published 2008-9
DOI 10.1094/MPMI-21-9-1175
PMID 18700822
MeSH Alphaproteobacteria / physiology Bradyrhizobium / physiology Gene Expression Regulation, Plant Hemoglobins / genetics* Host-Pathogen Interactions Lotus / genetics* Lotus / metabolism* Lotus / microbiology Microscopy, Fluorescence Nitric Oxide / metabolism* Plant Roots / genetics Plant Roots / metabolism Plant Roots / microbiology Reverse Transcriptase Polymerase Chain Reaction Sinorhizobium meliloti / physiology* Symbiosis / genetics Symbiosis / physiology
IF 3.649
Times Cited 36
WOS Category PLANT SCIENCES BIOTECHNOLOGY & APPLIED MICROBIOLOGY BIOCHEMISTRY & MOLECULAR BIOLOGY
Resource
Lotus / Glycine Miyakojima MG20