RRC ID 37824
Author Yasuda S, Kobayashi M, Itagaki S, Hirano T, Iseki K.
Title Response of the ABCG2 promoter in T47D cells and BeWo cells to sex hormone treatment.
Journal Mol Biol Rep
Abstract The aim of this study was to elucidate the effects of sex hormones on activity of the ABCG2 promoter in different cell lines. T47D cells and BeWo cells were used as models for ABCG2-expressing cell lines, and luciferase assays using ABCG2 promoter-luciferase constructs were performed. It was shown that progesterone increased the response of the ABCG2 promoter in T47D cells but not in BeWo cells. On the other hand, estradiol had no effect on response of the ABCG2 promoter in either cell line. However, response of the ABCG2 promoter was enhanced by overexpression of ERalpha in both T47D cells and BeWo cells. T47D cells had higher sensitivity to ERalpha than did BeWo cells. Furthermore, it was shown that the inductive effect of progesterone on the ABCG2 promoter was inhibited by addition of RU486 or mithramycin A. Therefore, it was thought that the ABCG2 promoter responded to stimulation of the progesterone receptor (PR)-Sp1 pathway in T47D cells. Furthermore, progesterone suppressed the response of the ABCG2 promoter by changing the expression levels of PR-A and PR-B in BeWo cells. These findings suggested that there are differences between cell lines in the regulation mechanism of ABCG2 expression by sex hormone treatment.
Volume 36(7)
Pages 1889-96
Published 2009-9-1
DOI 10.1007/s11033-008-9395-0
PMID 18991020
MeSH ATP Binding Cassette Transporter, Subfamily G, Member 2 ATP-Binding Cassette Transporters / genetics* Cell Line, Tumor Estradiol / pharmacology* Estrogen Receptor alpha / metabolism Humans Mifepristone / pharmacology Neoplasm Proteins / genetics* Plicamycin / analogs & derivatives Plicamycin / pharmacology Progesterone / pharmacology* Promoter Regions, Genetic* Receptors, Progesterone / metabolism Time Factors
IF 1.402
Times Cited 10
Human and Animal Cells