RRC ID 37831
著者 Yokoi H, Mizukami H, Nagatsu A, Ohno T, Tanabe H, Inoue M.
タイトル Peroxisome proliferator-activated receptor gamma ligands isolated from adlay seed (Coix lacryma-jobi L. var. ma-yuen STAPF.).
ジャーナル Biol Pharm Bull
Abstract Through screening for natural ligands against peroxisome proliferator-activated receptor gamma (PPARgamma) using the PPARgamma luciferase reporter assay, 6 hydroxy unsaturated fatty acids were isolated from adlay seed (Coix lacryma-jobi L. var. ma-yuen STAPF.) extracts with acetone and 70% ethanol. The structures of these compounds were determined via spectral analysis as 13-hydroxy-(9E,11E)-octadecadienoic acid (13-E,E-HODE) (1), 9-hydroxy-(10E,12E)-octadecadienoic acid (9-E,E-HODE) (2), 9-hydroxy-(10E)-octadecenoic acid (3), 10-hydroxy-(8E)-octadecenoic acid (4), 8-hydroxy-(9E)-octadecenoic acid (5), 11-hydroxy-(9Z)-octadecenoic acid (6). 9-E,E-HODE (2) exhibited the most potent PPARgamma agonist activity of the isolated hydroxy unsaturated fatty acids. 9-E,E-HODE (2) and 13-E,E-HODE (1) are the respective geometrical isomers of 9-hydroxy-(10E,12Z)-octadecadienoic acid and 13-hydroxy-(9Z,11E)-octadecadienoic acid, both of which are likely to be natural PPARgamma agonists produced in various mammalian cells, suggesting that 9-E,E-HODE may also act as PPARgamma agonist.
巻・号 32(4)
ページ 735-40
公開日 2009-4-1
DOI 10.1248/bpb.32.735
PII JST.JSTAGE/bpb/32.735
PMID 19336916
MeSH 3T3 Cells Adipocytes / drug effects Animals Cell Differentiation / drug effects Cell Line Coix / chemistry* Fatty Acids, Unsaturated / chemistry Fatty Acids, Unsaturated / isolation & purification Fatty Acids, Unsaturated / pharmacology Humans Ligands Linoleic Acids, Conjugated / pharmacology Luciferases / genetics Magnetic Resonance Spectroscopy Mice PPAR gamma / agonists* Plant Extracts / chemistry Rosiglitazone Seeds / chemistry Spectrometry, Mass, Electrospray Ionization Structure-Activity Relationship Thiazolidinediones / pharmacology
IF 1.863
引用数 12
WOS 分野 PHARMACOLOGY & PHARMACY
リソース情報
ヒト・動物細胞 293(RCB1637)