RRC ID 37842
Author Kuhara M, Yoshino T, Shiokawa M, Okabe T, Mizoguchi S, Yabuhara A, Takeyama H, Matsunaga T.
Title Magnetic separation of human podocalyxin-like protein 1 (hPCLP1)-positive cells from peripheral blood and umbilical cord blood using anti-hPCLP1 monoclonal antibody and protein A expressed on bacterial magnetic particles.
Journal Cell Struct. Funct.
Abstract Hemangioblasts are common progenitors of hematopoietic and angiogenic cells, which have been demonstrated in the mouse to possess a unique cell surface marker, podocalyxin-like protein 1 (PCLP1) (Hara, T. et al., Immunity, 11: 567-578. 1999). In this study, we prepared a novel monoclonal antibody against human PCLP1 (hPCLP1) and attempted to isolate human hematopoietic progenitor cells from umbilical cord blood and peripheral blood using nano-sized bacterial magnetic particles (BacMPs) coupled with the anti-hPCLP1 antibody. Flow cytometric analysis demonstrated that the purity of separated hPCLP1-positive cells from peripheral blood was approximately 95% whereas peripheral blood mononuclear cells contained only 0.1% PCLP1+ cells. Umbilical cord blood was demonstrated to be a better source for PCLP1+ cells than peripheral blood. These results suggest that the separation of human PCLP1+ cells using BacMPs with anti-hPCLP1 were extremely effective and may be useful as a means to prepare human hematopoietic progenitor cells.
Volume 34(1)
Pages 23-30
Published 2009
DOI 10.1247/csf.08043
PII JST.JSTAGE/csf/08043
PMID 19293534
MeSH Animals Antibodies, Monoclonal / metabolism Cell Line Fetal Blood / chemistry Flow Cytometry Hematopoietic Stem Cells / chemistry Hematopoietic Stem Cells / cytology Hematopoietic Stem Cells / immunology* Humans Immunomagnetic Separation / methods* Magnetospirillum / metabolism Mice Mice, Inbred BALB C Nanoparticles / chemistry Sialoglycoproteins / blood* Sialoglycoproteins / immunology* Sialoglycoproteins / isolation & purification Staphylococcal Protein A / immunology
IF 3.5
Times Cited 7
WOS Category CELL BIOLOGY
Resource
Human and Animal Cells