論文 - 詳細
| RRC ID | 37842 |
|---|---|
| 著者 | Kuhara M, Yoshino T, Shiokawa M, Okabe T, Mizoguchi S, Yabuhara A, Takeyama H, Matsunaga T. |
| タイトル | Magnetic separation of human podocalyxin-like protein 1 (hPCLP1)-positive cells from peripheral blood and umbilical cord blood using anti-hPCLP1 monoclonal antibody and protein A expressed on bacterial magnetic particles. |
| ジャーナル | Cell Struct Funct |
| Abstract |
Hemangioblasts are common progenitors of hematopoietic and angiogenic cells, which have been demonstrated in the mouse to possess a unique cell surface marker, podocalyxin-like protein 1 (PCLP1) (Hara, T. et al., Immunity, 11: 567-578. 1999). In this study, we prepared a novel monoclonal antibody against human PCLP1 (hPCLP1) and attempted to isolate human hematopoietic progenitor cells from umbilical cord blood and peripheral blood using nano-sized bacterial magnetic particles (BacMPs) coupled with the anti-hPCLP1 antibody. Flow cytometric analysis demonstrated that the purity of separated hPCLP1-positive cells from peripheral blood was approximately 95% whereas peripheral blood mononuclear cells contained only 0.1% PCLP1+ cells. Umbilical cord blood was demonstrated to be a better source for PCLP1+ cells than peripheral blood. These results suggest that the separation of human PCLP1+ cells using BacMPs with anti-hPCLP1 were extremely effective and may be useful as a means to prepare human hematopoietic progenitor cells. |
| 巻・号 | 34(1) |
| ページ | 23-30 |
| 公開日 | 2009-1-1 |
| DOI | 10.1247/csf.08043 |
| PII | JST.JSTAGE/csf/08043 |
| PMID | 19293534 |
| MeSH | Animals Antibodies, Monoclonal / metabolism Cell Line Fetal Blood / chemistry Flow Cytometry Hematopoietic Stem Cells / chemistry Hematopoietic Stem Cells / cytology Hematopoietic Stem Cells / immunology* Humans Immunomagnetic Separation / methods* Magnetospirillum / metabolism Mice Mice, Inbred BALB C Nanoparticles / chemistry Sialoglycoproteins / blood* Sialoglycoproteins / immunology* Sialoglycoproteins / isolation & purification Staphylococcal Protein A / immunology |
| IF | 2.429 |
| 引用数 | 9 |
| WOS 分野 | CELL BIOLOGY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | Patent(IFI CLAIMS) |
| 各媒体での言及数の合計 | 2 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| ヒト・動物細胞 | OP9(RCB1124) |