RRC ID |
37842
|
著者 |
Kuhara M, Yoshino T, Shiokawa M, Okabe T, Mizoguchi S, Yabuhara A, Takeyama H, Matsunaga T.
|
タイトル |
Magnetic separation of human podocalyxin-like protein 1 (hPCLP1)-positive cells from peripheral blood and umbilical cord blood using anti-hPCLP1 monoclonal antibody and protein A expressed on bacterial magnetic particles.
|
ジャーナル |
Cell Struct Funct
|
Abstract |
Hemangioblasts are common progenitors of hematopoietic and angiogenic cells, which have been demonstrated in the mouse to possess a unique cell surface marker, podocalyxin-like protein 1 (PCLP1) (Hara, T. et al., Immunity, 11: 567-578. 1999). In this study, we prepared a novel monoclonal antibody against human PCLP1 (hPCLP1) and attempted to isolate human hematopoietic progenitor cells from umbilical cord blood and peripheral blood using nano-sized bacterial magnetic particles (BacMPs) coupled with the anti-hPCLP1 antibody. Flow cytometric analysis demonstrated that the purity of separated hPCLP1-positive cells from peripheral blood was approximately 95% whereas peripheral blood mononuclear cells contained only 0.1% PCLP1+ cells. Umbilical cord blood was demonstrated to be a better source for PCLP1+ cells than peripheral blood. These results suggest that the separation of human PCLP1+ cells using BacMPs with anti-hPCLP1 were extremely effective and may be useful as a means to prepare human hematopoietic progenitor cells.
|
巻・号 |
34(1)
|
ページ |
23-30
|
公開日 |
2009-1-1
|
DOI |
10.1247/csf.08043
|
PII |
JST.JSTAGE/csf/08043
|
PMID |
19293534
|
MeSH |
Animals
Antibodies, Monoclonal / metabolism
Cell Line
Fetal Blood / chemistry
Flow Cytometry
Hematopoietic Stem Cells / chemistry
Hematopoietic Stem Cells / cytology
Hematopoietic Stem Cells / immunology*
Humans
Immunomagnetic Separation / methods*
Magnetospirillum / metabolism
Mice
Mice, Inbred BALB C
Nanoparticles / chemistry
Sialoglycoproteins / blood*
Sialoglycoproteins / immunology*
Sialoglycoproteins / isolation & purification
Staphylococcal Protein A / immunology
|
IF |
2.429
|
引用数 |
9
|
WOS 分野
|
CELL BIOLOGY
|
リソース情報 |
ヒト・動物細胞 |
OP9(RCB1124) |