論文 - 詳細
| RRC ID | 37843 |
|---|---|
| 著者 | Kim CH, Yoo IeR, Chung YA, Park YH, Kim SH, Sohn HS, Chung SK. |
| タイトル | Influence of thyroid-stimulating hormone on 18F-fluorodeoxyglucose and 99mTc-methoxyisobutylisonitrile uptake in human poorly differentiated thyroid cancer cells in vitro. |
| ジャーナル | Ann Nucl Med |
| Abstract |
OBJECTIVE:In poorly differentiated thyroid cancer originating from thyroid follicular cells, the ability to concentrate iodine is lost. This makes recurrence undetectable by (131)I whole-body scan. In this situation, other radiopharmaceuticals, such as (18)F-fluorodeoxyglucose ((18)F-FDG) and technetium-99m-methoxyisobutylisonitrile ((99m)Tc-MIBI), are used to evaluate recurrence or metastasis. Some reports suggest that (18)F-FDG uptake is increased by thyroid-stimulating hormone (TSH) stimulation. This study aimed to determine the influence of TSH on (18)F-FDG and (99m)Tc-MIBI uptake in human poorly differentiated thyroid cancer cells in vitro. MATERIALS AND METHODS:The cells were stimulated with 1000 muU/ml of recombinant human thyroid-stimulating hormone (rhTSH) for 1 day, 3 days, and 5 days. Each cell was incubated with 0.5 MBq/ml-1 MBq/ml of (18)F-FDG or 0.5 MBq/ml-1 MBq/ml of (99m)Tc-MIBI for 1 h at 37 degrees C. The uptake of each radiopharmaceutical in the cells was quantified as a percent of whole radioactivity per total viable cell number. The quantification of glucose transporter 1, 2, 3 and 4 mRNA expression was measured using RT-PCR. RESULTS:TSH stimulation increased (18)F-FDG uptake in a time-dependent manner. Following 5 days of rhTSH stimulation, (18)F-FDG uptake was approximately 2.2 times that of the control. The increase in (18)F-FDG uptake following rhTSH stimulation was correlated to the increase in GLUT4 mRNA level. The GLUT1 mRNA level was unchanged. An increased uptake of (99m)Tc-MIBI was observed with a pattern similar to that of (18)F-FDG. The (99m)Tc-MIBI uptake was approximately 1.5 times that of the control 5 days later. CONCLUSIONS:These results suggest that TSH stimulates (18)F-FDG and (99m)Tc-MIBI uptake in poorly differentiated papillary thyroid cancer, and therefore (18)F-FDG-PET or (99m)Tc-MIBI scans under TSH stimulation may be more accurate than under suppression. |
| 巻・号 | 23(2) |
| ページ | 131-6 |
| 公開日 | 2009-2-1 |
| DOI | 10.1007/s12149-008-0218-0 |
| PMID | 19225935 |
| MeSH | Adenocarcinoma, Follicular / diagnostic imaging Adenocarcinoma, Follicular / metabolism* Adenocarcinoma, Follicular / pathology Cell Differentiation Fluorodeoxyglucose F18 / pharmacokinetics* Humans Metabolic Clearance Rate / drug effects Radionuclide Imaging Technetium Tc 99m Sestamibi / pharmacokinetics* Thyroid Neoplasms / diagnostic imaging Thyroid Neoplasms / metabolism* Thyroid Neoplasms / pathology Thyrotropin / administration & dosage* Tumor Cells, Cultured |
| IF | 2.607 |
| 引用数 | 8 |
| WOS 分野 | RADIOLOGY, NUCLEAR MEDICINE & MEDICAL IMAGING |
| リソース情報 | |
| ヒト・動物細胞 | |