| RRC ID |
37849
|
| 著者 |
Susa T, Ishikawa A, Kato T, Nakayama M, Kitahara K, Kato Y.
|
| タイトル |
Regulation of porcine pituitary glycoprotein hormone alpha subunit gene with LIM-homeobox transcription factor Lhx3.
|
| ジャーナル |
J Reprod Dev
|
| Abstract |
The aim of this study was to characterize the promoter activity of the porcine pituitary glycoprotein hormone common alpha gene (Cga) promoter (-1059/+12) and the role of LIM homeodomain transcription factor Lhx3. A transfection assay using Chinese hamster ovary (CHO) cells showed that the -1059/-101 region of the Cga promoter definitely responds to Lhx3 and that the -1059/-240 region exhibits a high basal transcriptional level in a pituitary-derived cell line, LbetaT2. A DNA binding and DNase I footprinting assay demonstrated that Lhx3 has seven binding sites in the -1059/+12 region of Cga, including a pituitary glycoprotein hormone basal element (PGBE) known as a LIM homeodomain factor-binding site. A transfection assay of the sequence of Lhx3-binding sites fused with minimal promoter vector confirmed their Lhx3-dependent stimulations in LbetaT2 cells. RT-PCR analysis of porcine pituitary ontogeny demonstrated that porcine Lhx3 showed striking changes of expression in both sexes during the fetal period but a stable high level of expression after birth. Thus, the porcine Cga promoter is regulated by Lhx3 through seven sites in the distal and proximal regions.
|
| 巻・号 |
55(4)
|
| ページ |
425-32
|
| 公開日 |
2009-8-1
|
| DOI |
10.1262/jrd.20232
|
| PII |
JST.JSTAGE/jrd/20232
|
| PMID |
19444006
|
| MeSH |
Animals
Binding Sites
CHO Cells
Cell Line
Cell Line, Tumor
Cricetinae
Cricetulus
DNA / metabolism
Deoxyribonuclease I / metabolism
Gene Expression Regulation*
Glycoprotein Hormones, alpha Subunit / biosynthesis*
Glycoprotein Hormones, alpha Subunit / genetics*
Homeodomain Proteins / biosynthesis*
LIM-Homeodomain Proteins
Mice
Protein Structure, Tertiary
Swine
Transcription Factors
|
| IF |
1.735
|
| 引用数 |
7
|
|
WOS 分野
|
AGRICULTURE, DAIRY & ANIMAL SCIENCE
REPRODUCTIVE BIOLOGY
|
| リソース情報 |
| ヒト・動物細胞 |
CHO-K1(RCB0285) |
