RRC ID 3785
Author Shimoda Y, Nagata M, Suzuki A, Abe M, Sato S, Kato T, Tabata S, Higashi S, Uchiumi T.
Title Symbiotic rhizobium and nitric oxide induce gene expression of non-symbiotic hemoglobin in Lotus japonicus.
Journal Plant Cell Physiol.
Abstract We characterized the expression profiles of LjHb1 and LjHb2, non-symbiotic hemoglobin (non-sym-Hb) genes of Lotus japonicus. Although LjHb1 and LjHb2 showed 77% homology in their cDNA sequences, LjHb2 is located in a unique position in the phylogenetic tree of plant Hbs. The 5'-upstream regions of both genes contain the motif AAAGGG at a position similar to that in promoters of other non-sym-Hb genes. Expression profiles obtained by using quantitative RT-PCR showed that LjHb1 and LjHb2 were expressed in all tissues of mature plants, and expression was enhanced in mature root nodules. LjHb1 was strongly induced under both hypoxic and cold conditions, and by the application of nitric oxide (NO) donor, whereas LjHb2 was induced only by the application of sucrose. LjHb1 was also induced transiently by the inoculation with the symbiotic rhizobium Mesorhizobium loti MAFF303099. Observations using fluorescence microscopy revealed the induction of LjHb1 expression corresponded to the generation of NO. These results suggest that non-sym-Hb and NO have important roles in stress adaptation and in the early stage of legume-rhizobium symbiosis.
Volume 46(1)
Pages 99-107
Published 2005-1
DOI 10.1093/pci/pci001
PII pci001
PMID 15668209
MeSH Alphaproteobacteria / metabolism* Base Sequence DNA, Plant / genetics Gene Expression Gene Expression Regulation, Plant / drug effects Genes, Plant Hemoglobins / genetics* Lotus / drug effects Lotus / genetics* Lotus / metabolism Lotus / microbiology Nitric Oxide / metabolism* Nitric Oxide Donors / pharmacology Phylogeny Plant Growth Regulators / pharmacology Plant Proteins / genetics Promoter Regions, Genetic Symbiosis
IF 3.929
Times Cited 73
Lotus / Glycine Miyakojima MG20