Reference - Detail
| RRC ID | 37854 |
|---|---|
| Author | Kobayashi H, Nishikawa M, Sakamoto C, Nishio T, Kanazawa H, Okano T. |
| Title | Dual temperature- and pH-responsive fluorescence molecular probe for cellular imaging utilizing a PNIPAAm-fluorescein copolymer. |
| Journal | Anal Sci |
| Abstract |
The thermally controlling fluorescence intensity of a molecular probe for cellular imaging has been investigated. A reversible temperature-induced phase transition of N-isopropylacrylamide/fluorescein copolymers [poly(NIPAAm-co-FL)] was used as a molecular switch to control the fluorescence intensity of the imaging probe. The copolymer displayed environmentally sensitive fluorescence properties, in which the fluorescence intensity changed with the response to both the temperature and the pH. Utilizing these features, we monitored the thermal-aggregation process of BSA by the fluorescence resonance energy transfer (FRET) method. Additionally, the cellular uptake in RAW264.7 cells of poly(NIPAAm-co-FL) conjugated with lipid was studied using a confocal laser scanning microscope. |
| Volume | 25(8) |
| Pages | 1043-7 |
| Published | 2009-8-1 |
| DOI | 10.2116/analsci.25.1043 |
| PII | JST.JSTAGE/analsci/25.1043 |
| PMID | 19667485 |
| MeSH | Acrylic Resins / chemistry* Acrylic Resins / metabolism Animals Cell Line Fluoresceins / chemistry* Fluoresceins / metabolism Fluorescence Resonance Energy Transfer Hydrogen-Ion Concentration Lipids / chemistry Molecular Probes / chemistry* Molecular Probes / metabolism Nanotechnology Rats Serum Albumin, Bovine / metabolism* Temperature |
| IF | 2.049 |
| Times Cited | 14 |
| WOS Category | CHEMISTRY, ANALYTICAL |
| Altmetric score |
オルトメトリクス指標項目
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| The most frequently cited source | Patent(IFI CLAIMS) |
| Total number of mentions | 1 |
| Altmetric score changes over past 6months | 0.0 |
| Resource | |
| Human and Animal Cells | RAW 264(RCB0535) |