Reference - Detail
| RRC ID | 37885 |
|---|---|
| Author | Aini H, Itaka K, Fujisawa A, Uchida H, Uchida S, Fukushima S, Kataoka K, Saito T, Chung UI, Ohba S. |
| Title | Messenger RNA delivery of a cartilage-anabolic transcription factor as a disease-modifying strategy for osteoarthritis treatment. |
| Journal | Sci Rep |
| Abstract |
Osteoarthritis (OA) is a chronic degenerative joint disease and a major health problem in the elderly population. No disease-modifying osteoarthritis drug (DMOAD) has been made available for clinical use. Here we present a disease-modifying strategy for OA, focusing on messenger RNA (mRNA) delivery of a therapeutic transcription factor using polyethylene glycol (PEG)-polyamino acid block copolymer-based polyplex nanomicelles. When polyplex nanomicelles carrying the cartilage-anabolic, runt-related transcription factor (RUNX) 1 mRNA were injected into mouse OA knee joints, OA progression was significantly suppressed compared with the non-treatment control. Expressions of cartilage-anabolic markers and proliferation were augmented in articular chondrocytes of the RUNX1-injected knees. Thus, this study provides a proof of concept of the treatment of degenerative diseases such as OA by the in situ mRNA delivery of therapeutic transcription factors; the presented approach will directly connect basic findings on disease-protective or tissue-regenerating factors to disease treatment. |
| Volume | 6 |
| Pages | 18743 |
| Published | 2016-1-5 |
| DOI | 10.1038/srep18743 |
| PII | srep18743 |
| PMID | 26728350 |
| PMC | PMC4700530 |
| MeSH | Animals Cartilage, Articular / metabolism* Cartilage, Articular / pathology Disease Models, Animal Gene Expression Gene Transfer Techniques Genes, Reporter Injections, Intra-Articular Mice Micelles Nanocomposites / administration & dosage Nanocomposites / chemistry Osteoarthritis / genetics* Osteoarthritis / metabolism* Osteoarthritis / pathology Osteoarthritis / therapy Osteoarthritis, Knee / genetics Osteoarthritis, Knee / metabolism Osteoarthritis, Knee / pathology Osteoarthritis, Knee / therapy RNA, Messenger / administration & dosage* RNA, Messenger / chemistry RNA, Messenger / genetics* Transcription Factors / genetics* Transcription Factors / metabolism |
| IF | 3.998 |
| Times Cited | 38 |
| WOS Category | RHEUMATOLOGY |
| Altmetric score |
オルトメトリクス指標項目
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| The most frequently cited source | X(Twitter) |
| Total number of mentions | 27 |
| Altmetric score changes over past 6months | 0.0 |
| Resource | |
| Human and Animal Cells | HeLa(RCB0007) 293(RCB1637) |