論文 - 詳細
| RRC ID | 37891 |
|---|---|
| 著者 | Miura S, Sato K, Kato-Negishi M, Teshima T, Takeuchi S. |
| タイトル | Fluid shear triggers microvilli formation via mechanosensitive activation of TRPV6. |
| ジャーナル | Nat Commun |
| Abstract |
Microvilli are cellular membrane protrusions present on differentiated epithelial cells, which can sense and interact with the surrounding fluid environment. Biochemical and genetic approaches have identified a set of factors involved in microvilli formation; however, the underlying extrinsic regulatory mechanism of microvilli formation remains largely unknown. Here we demonstrate that fluid shear stress (FSS), an external mechanical cue, serves as a trigger for microvilli formation in human placental trophoblastic cells. We further reveal that the transient receptor potential, vanilloid family type-6 (TRPV6) calcium ion channel plays a critical role in flow-induced Ca(2+) influx and microvilli formation. TRPV6 regulates phosphorylation of Ezrin via a Ca(2+)-dependent phosphorylation of Akt; this molecular event is necessary for microvillar localization of Ezrin in response to FSS. Our findings provide molecular insight into the microvilli-mediated mechanoresponsive cellular functions, such as epithelial absorption, signal perception and mechanotransduction. |
| 巻・号 | 6 |
| ページ | 8871 |
| 公開日 | 2015-11-13 |
| DOI | 10.1038/ncomms9871 |
| PII | ncomms9871 |
| PMID | 26563429 |
| PMC | PMC4660203 |
| MeSH | Calcium / metabolism Calcium Channels / genetics Calcium Channels / metabolism* Cytoskeletal Proteins / genetics Cytoskeletal Proteins / metabolism Humans Mechanotransduction, Cellular* Microvilli / chemistry Microvilli / metabolism* Phosphorylation TRPV Cation Channels / genetics TRPV Cation Channels / metabolism* Trophoblasts / metabolism |
| IF | 12.121 |
| 引用数 | 50 |
| WOS 分野 | CELL BIOLOGY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | News |
| 各媒体での言及数の合計 | 12 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| ヒト・動物細胞 | BeWo(RCB1644) |