論文 - 詳細
| RRC ID | 37899 |
|---|---|
| 著者 | Takayama S, Hashimoto K, Kokubu E, Taniguchi M, Tajima K, Ochiai A, Saitoh E, Saito A, Ishihara K, Kato T. |
| タイトル | Inhibitory effects of a novel cationic dodecapeptide [CL(14-25)] derived from cyanate lyase of rice on endotoxic activities of LPSs from Escherichia coli and periodontopathic Aggregatibacter actinomycetemcomitans. |
| ジャーナル | Microb Pathog |
| Abstract |
OBJECTIVE:CL(14-25), a dodecapeptide of cyanate lyase from rice, is a novel cationic α-helical antimicrobial peptide. In this study, we examined inhibitory ability of CL(14-25) against endotoxic activities of lipopolysaccharides (LPSs) from Escherichia coli and periodontal pathogenic Aggregatibacter actinomycetemcomitans. METHODS:Endotoxin-neutralizing activity of CL(14-25) was evaluated by inhibition to induction of cytokine and nitric oxide in human aortic endothelial cells (HAECs) and RAW264 mouse macrophage cells, respectively. Protective effect of CL(14-25) was determined in mice against lethal toxicity of LPS. RESULTS:IL-6 in HAECs was induced by stimulation with LPS preparations of A. actinomycetemcomitans and E. coli tested in this study, and addition of CL(14-25) to the medium caused inhibition of their induction in a dose-dependent manner. CL(14-25) inhibited NO induction in RAW264 cells by a smooth type LPS of E. coli O55:B5 and an Rc type LPS of E. coli J5 as well as lipid A of E. coli R515 in a dose-dependent manner. Simultaneous injection of E. coli O55:B5 LPS and CL(14-25) in BALB/c mice resulted in prevention of lethal toxicity of the former. The results of a Limulus amebocyte lysate assay and surface plasmon resonance analysis of interaction between CL(14-25) and E. coli LPS or lipid A showed that CL(14-25) specifically binds to a lipid A moiety of LPS. CONCLUSION:The results of present study suggest that CL(14-25) has a potential to be used as a nutraceutical agent for periodontal therapy. |
| 巻・号 | 94 |
| ページ | 2-11 |
| 公開日 | 2016-5-1 |
| DOI | 10.1016/j.micpath.2015.08.011 |
| PII | S0882-4010(15)00142-4 |
| PMID | 26318142 |
| MeSH | Aggregatibacter actinomycetemcomitans / chemistry Aggregatibacter actinomycetemcomitans / metabolism* Animals Carbon-Nitrogen Lyases / chemistry* Cytokines / biosynthesis Cytokines / metabolism Dose-Response Relationship, Drug Drug Interactions Endothelial Cells / drug effects Escherichia coli / chemistry Escherichia coli / metabolism* Humans Interleukin-6 / biosynthesis Lipid A / antagonists & inhibitors Lipid A / chemistry Lipid A / toxicity Lipopolysaccharides / antagonists & inhibitors* Lipopolysaccharides / chemistry Lipopolysaccharides / toxicity Macrophages / drug effects Macrophages / metabolism Male Mice Mice, Inbred BALB C Nitric Oxide / biosynthesis Nitric Oxide / metabolism Oryza / enzymology Peptide Fragments / chemistry Peptide Fragments / pharmacology* RAW 264.7 Cells |
| IF | 2.914 |
| 引用数 | 6 |
| WOS 分野 | MICROBIOLOGY IMMUNOLOGY |
| リソース情報 | |
| ヒト・動物細胞 | RAW 264(RCB0535) |