RRC ID 37901
著者 Ozeki M, Salah A, Aini W, Tamaki K, Haga H, Miyagawa-Hayashino A.
タイトル Abnormal Localization of STK17A in Bile Canaliculi in Liver Allografts: An Early Sign of Chronic Rejection.
ジャーナル PLoS One
Abstract The biological significance of STK17A, a serine/threonine kinase, in the liver is not known. We analyzed STK17A expression in HepG2 cells and human liver tissue. Accordingly, we investigated whether STK17A could help in identifying earlier changes during the evolution of chronic rejection (CR) after liver transplantation. RT-PCR and immunofluorescence were used to analyze STK17A expression in HepG2 cells. Antibody microarray was performed using human liver samples from CR and healthy donors. Immunohistochemistry was used to verify the clinical utility of STK17A on sequential biopsies for the subsequent development of CR. A novel short isoform of STK17A was found in HepG2 cells. STK17A was localized in the nuclei and bile canaliculi in HepG2 cells and human livers. Microarray of STK17A revealed its decrease in failed liver allografts by CR. During the evolution of CR, the staining pattern of bile canalicular STK17A gradually changed from diffuse linear to focal intermittent. The focal intermittent staining pattern was observed before the definite diagnosis of CR. In conclusion, the present study was the first to find localization of STK17A in normal bile canaliculi. Abnormal expression and localization of STK17A were associated with CR of liver allografts since the early stage of the rejection process.
巻・号 10(8)
ページ e0136381
公開日 2015-1-1
DOI 10.1371/journal.pone.0136381
PII PONE-D-15-21368
PMID 26305096
PMC PMC4549187
MeSH ATP Binding Cassette Transporter, Subfamily B, Member 11 ATP-Binding Cassette Transporters / metabolism Allografts / metabolism* Amino Acid Sequence Apoptosis Regulatory Proteins / chemistry Apoptosis Regulatory Proteins / genetics Apoptosis Regulatory Proteins / metabolism* Base Sequence Bile Canaliculi / metabolism* Biopsy Demography Female Graft Rejection / metabolism* Hep G2 Cells Humans Immunohistochemistry Keratin-7 / metabolism Liver Transplantation* Male Molecular Sequence Data Protein Isoforms / metabolism Protein Serine-Threonine Kinases / chemistry Protein Serine-Threonine Kinases / genetics Protein Serine-Threonine Kinases / metabolism* Protein Transport Tissue Donors
IF 2.74
引用数 4
WOS 分野 SURGERY
リソース情報
ヒト・動物細胞 Hep G2(RCB1886)