論文 - 詳細
| RRC ID | 37911 |
|---|---|
| 著者 | Okuda K, Ito A, Uehara T. |
| タイトル | Regulation of Histone Deacetylase 6 Activity via S-Nitrosylation. |
| ジャーナル | Biol Pharm Bull |
| Abstract |
Nitric oxide (NO) is a gaseous regulatory factor produced by NO synthases (NOS) and it plays several critical roles via S-nitrosylation of protein cysteine residues. Histone deacetylase (HDAC) functions in the maintenance/balance of chromatin acetylation and contributes to transcriptional supression. It has been reported that S-nitrosylation of HDAC2 is involved in the regulation of deacetylase activity. However, it remains unknown whether other subtypes of the HDAC family are S-nitrosylated. In the present study, we found that HDAC6 is a target of NO. A biotin-switch assay revealed that endogenous HDAC6 is S-nitrosylated by both NO donors and NO derived from the inducible type of NOS in cells treated with cytokines. NO led to suppressed deacetylase activity in vitro and increased acetylated α-tubulin, a major substrate for HDAC6, in A549 cells. These findings suggest that S-nitrosylation of HDAC6 plays a pivotal role in the regulation of protein acetylation. |
| 巻・号 | 38(9) |
| ページ | 1434-7 |
| 公開日 | 2015-1-1 |
| DOI | 10.1248/bpb.b15-00364 |
| PMID | 26328501 |
| MeSH | Acetylation Cell Line, Tumor Cysteine / analogs & derivatives Cysteine / pharmacology Histone Deacetylase 6 Histone Deacetylases / metabolism* Humans Nitric Oxide / metabolism* Nitric Oxide Donors / pharmacology Nitric Oxide Synthase Type II / metabolism S-Nitrosothiols / pharmacology |
| IF | 1.863 |
| 引用数 | 14 |
| WOS 分野 | PHARMACOLOGY & PHARMACY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | X(Twitter) |
| 各媒体での言及数の合計 | 1 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| ヒト・動物細胞 | A549 |