論文 - 詳細
| RRC ID | 37914 |
|---|---|
| 著者 | Abe N, Matsumoto K, Nishihara M, Nakano Y, Shibata A, Maruyama H, Shuto S, Matsuda A, Yoshida M, Ito Y, Abe H. |
| タイトル | Rolling Circle Translation of Circular RNA in Living Human Cells. |
| ジャーナル | Sci Rep |
| Abstract |
We recently reported that circular RNA is efficiently translated by a rolling circle amplification (RCA) mechanism in a cell-free Escherichia coli translation system. Recent studies have shown that circular RNAs composed of exonic sequences are abundant in human cells. However, whether these circular RNAs can be translated into proteins within cells remains unclear. In this study, we prepared circular RNAs with an infinite open reading frame and tested their translation in eukaryotic systems. Circular RNAs were translated into long proteins in rabbit reticulocyte lysate in the absence of any particular element for internal ribosome entry, a poly-A tail, or a cap structure. The translation systems in eukaryote can accept much simpler RNA as a template for protein synthesis by cyclisation. Here, we demonstrated that the circular RNA is efficiently translated in living human cells to produce abundant protein product by RCA mechanism. These findings suggest that translation of exonic circular RNAs present in human cells is more probable than previously thought. |
| 巻・号 | 5 |
| ページ | 16435 |
| 公開日 | 2015-11-10 |
| DOI | 10.1038/srep16435 |
| PII | srep16435 |
| PMID | 26553571 |
| PMC | PMC4639774 |
| MeSH | Animals Cell Line Cell-Free System Genes, Reporter HeLa Cells Humans In Vitro Techniques Protein Biosynthesis* RNA* / chemical synthesis RNA, Circular RNA, Messenger* Rabbits |
| IF | 3.998 |
| 引用数 | 122 |
| WOS 分野 | BIOCHEMISTRY & MOLECULAR BIOLOGY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | Patent(IFI CLAIMS) |
| 各媒体での言及数の合計 | 25 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| ヒト・動物細胞 | HeLa(RCB0007) |