Reference - Detail
| RRC ID | 3805 |
|---|---|
| Author | Takeda S, Tsukiji S, Ueda H, Nagamune T. |
| Title | Covalent split protein fragment-DNA hybrids generated through N-terminus-specific modification of proteins by oligonucleotides. |
| Journal | Org Biomol Chem |
| Abstract |
Semisynthetic protein-DNA hybrid molecules have recently attracted much attention as valuable tools for bioanalytical chemistry and nanobiotechnology. Here we describe a synthetic method for conjugating oligonucleotides to the N-terminus of recombinant proteins. Our strategy involves the conversion of amine-terminated oligonucleotides to thioester-functionalized oligonucleotides by using a bifunctional reagent bearing an N-hydroxysuccinimide ester and benzyl thioester group, followed by native chemical ligation with proteins containing an N-terminal cysteine. We applied this technique to construct split luciferase fragment-DNA hybrid systems in which the catalytic activity of split luciferase is restored by the re-assembly of each fragment through a specific DNA-protein or DNA-DNA interaction. Split protein fragment-DNA hybrids will offer new opportunities to explore the potential of protein-DNA conjugates for various applications. |
| Volume | 6(12) |
| Pages | 2187-94 |
| Published | 2008-6-21 |
| DOI | 10.1039/b720013g |
| PMID | 18528581 |
| MeSH | Base Sequence Chromatography, High Pressure Liquid Oligonucleotides / chemistry* Proteins / chemistry* Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization |
| IF | 3.412 |
| Times Cited | 19 |
| WOS Category | CHEMISTRY, ORGANIC |
| Altmetric score |
オルトメトリクス指標項目
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| The most frequently cited source | Patent(IFI CLAIMS) |
| Total number of mentions | 6 |
| Altmetric score changes over past 6months | 0.0 |
| Resource | |
| DNA material | ETR103 (RDB01198) |