Reference - Detail
| RRC ID | 38093 |
|---|---|
| Author | Kwak HS, Kim JY, Sim SJ. |
| Title | A Microreactor System for Cultivation of Haematococcus pluvialis and Astaxanthin Production. |
| Journal | J Nanosci Nanotechnol |
| Abstract |
Development of efficient culture and monitoring system for cell growth and production of useful materials is required for practical utilization of microalgae. In the present study, we developed a PDMS-based microreactor system for efficient, rapid culture of microalgae and monitoring of cell growth, carotenoid content under diverse culture conditions. Due to advantages of PDMS, we optimized culture conditions (light intensity, pH, nitrate depletion, carbon dioxide concentration) for improving growth rate and astaxanthin productivity in considerably less time compared to conventional culture methods using flask or well plate. In addition, we found that there was a strong linear correlation between fluorescence intensity of astaxanthin stained by Nile red and the astaxanthin content, which can be utilized as a high-throughput screening tool in microfluidic systems. In this study, the growth rate of vegetative Haematococcus pluvialis was improved by 60% in microfluidic chamber than in flask and astaxanthin was produced up to 362 mg/L under the optimal conditions (300 µmol photon/m2/s of light, 7% CO2 (v/v), and pH 7.0) using designed microfluidic devices. This result shows that microfluidic system can provide effective means to address development of microalgal strains including H. pluvialis and bioprocess. |
| Volume | 15(2) |
| Pages | 1618-23 |
| Published | 2015-2-1 |
| DOI | 10.1166/jnn.2015.9321 |
| PMID | 26353702 |
| MeSH | Batch Cell Culture Techniques / instrumentation* Bioreactors / microbiology* Equipment Design Equipment Failure Analysis Lab-On-A-Chip Devices* Miniaturization Volvocida / cytology Volvocida / physiology* Xanthophylls / biosynthesis Xanthophylls / isolation & purification |
| IF | 1.134 |
| Times Cited | 7 |
| WOS Category | PHYSICS, APPLIED PHYSICS, CONDENSED MATTER MATERIALS SCIENCE, MULTIDISCIPLINARY CHEMISTRY, MULTIDISCIPLINARY NANOSCIENCE & NANOTECHNOLOGY |
| Altmetric score |
オルトメトリクス指標項目
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| The most frequently cited source | Patent(IFI CLAIMS) |
| Total number of mentions | 2 |
| Altmetric score changes over past 6months | 0.0 |
| Resource | |
| Algae | NIES-144 |