論文 - 詳細
| RRC ID | 38096 |
|---|---|
| 著者 | Darienko T, Gustavs L, Eggert A, Wolf W, Pröschold T. |
| タイトル | Evaluating the Species Boundaries of Green Microalgae (Coccomyxa, Trebouxiophyceae, Chlorophyta) Using Integrative Taxonomy and DNA Barcoding with Further Implications for the Species Identification in Environmental Samples. |
| ジャーナル | PLoS One |
| Abstract |
Integrative taxonomy is an approach for defining species and genera by taking phylogenetic, morphological, physiological, and ecological data into account. This approach is appropriate for microalgae, where morphological convergence and high levels of morphological plasticity complicate the application of the traditional classification. Although DNA barcode markers are well-established for animals, fungi, and higher plants, there is an ongoing discussion about suitable markers for microalgae and protists because these organisms are genetically more diverse compared to the former groups. To solve these problems, we assess the usage of a polyphasic approach combining phenotypic and genetic parameters for species and generic characterization. The application of barcode markers for database queries further allows conclusions about the 'coverage' of culture-based approaches in biodiversity studies and integrates additional aspects into modern taxonomic concepts. Although the culture-dependent approach revealed three new lineages, which are described as new species in this paper, the culture-independent analyses discovered additional putative new species. We evaluated three barcode markers (V4, V9 and ITS-2 regions, nuclear ribosomal operon) and studied the morphological and physiological plasticity of Coccomyxa, which became a model organism because its whole genome sequence has been published. In addition, several biotechnological patents have been registered for Coccomyxa. Coccomyxa representatives are distributed worldwide, are free-living or in symbioses, and colonize terrestrial and aquatic habitats. We investigated more than 40 strains and reviewed the biodiversity and biogeographical distribution of Coccomyxa species using DNA barcoding. The genus Coccomyxa formed a monophyletic group within the Trebouxiophyceae separated into seven independent phylogenetic lineages representing species. Summarizing, the combination of different characteristics in an integrative approach helps to evaluate environmental data and clearly identifies microalgae at generic and species levels. |
| 巻・号 | 10(6) |
| ページ | e0127838 |
| 公開日 | 2015-1-1 |
| DOI | 10.1371/journal.pone.0127838 |
| PII | PONE-D-15-04716 |
| PMID | 26080086 |
| PMC | PMC4469705 |
| MeSH | Biodiversity Chlorophyta / classification Chlorophyta / genetics* Chlorophyta / growth & development Classification / methods DNA Barcoding, Taxonomic* Genetic Markers Genetic Variation Microalgae / classification Microalgae / genetics* Microalgae / growth & development Phylogeny Species Specificity |
| IF | 2.74 |
| 引用数 | 56 |
| WOS 分野 | EVOLUTIONARY BIOLOGY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | X(Twitter) |
| 各媒体での言及数の合計 | 5 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| 藻類 | NIES-2252 NIES-2353 |