RRC ID 3813
Author Morita Y, Matsuyama H, Serizawa A, Takeya T, Kawakami H.
Title Identification of angiogenin as the osteoclastic bone resorption-inhibitory factor in bovine milk.
Journal Bone
Abstract We identified, for the first time, the factor responsible for inhibiting osteoclast-mediated bone resorption in the basic protein fraction of bovine milk (milk basic protein, MBP). The protein was purified by a combination of ion and gel column chromatography from MBP, based on its activity to prevent unfractionated rabbit bone cells from forming pits on dentine slices. It was found to have a molecular weight of 15 kDa on SDS-PAGE, and the sequence of the N-terminal 25 amino acid residues was identical to that of bovine angiogenin. The purified bovine angiogenin inhibited the pit-forming activity of both unfractionated bone cells and purified osteoclasts in a dose-dependent manner, and the inhibitory activity was markedly suppressed by treatment with anti-bovine angiogenin antibody. The inhibitory activity was confirmed in mice both in vitro and in vivo. Treatment of osteoclasts with bovine angiogenin resulted in an impairment of the formation F-actin ring and a reduction in the mRNA levels of TRAP and cathepsin K, both known to be essential for the bone resorption activity of osteoclasts. These results suggest that bovine angiogenin is the substance mainly responsible for the inhibitory effect of bovine milk on osteoclast-mediated bone resorption, and that it exerts its activity by acting directly on the osteoclasts.
Volume 42(2)
Pages 380-7
Published 2008-2
DOI 10.1016/j.bone.2007.10.012
PII S8756-3282(07)00777-6
PMID 18055286
MeSH Animals Base Sequence Bone Resorption / metabolism Bone Resorption / pathology* Conserved Sequence Female Gene Expression Regulation / drug effects Mice Microscopy, Electron, Scanning Milk / chemistry* Milk Proteins / chemistry Milk Proteins / isolation & purification Milk Proteins / pharmacology Molecular Sequence Data Osteoclasts / drug effects* Osteoclasts / metabolism Ribonuclease, Pancreatic / chemistry Ribonuclease, Pancreatic / isolation & purification Ribonuclease, Pancreatic / pharmacology* Sequence Alignment
IF 4.455
Times Cited 17
DNA material pCAhMCSF (RDB01524)