論文 - 詳細
| RRC ID | 38146 |
|---|---|
| 著者 | Kawamori Y, Katayama Y, Asada N, Minagawa K, Sato M, Okamura A, Shimoyama M, Nakagawa K, Okano T, Tanimoto M, Kato S, Matsui T. |
| タイトル | Role for vitamin D receptor in the neuronal control of the hematopoietic stem cell niche. |
| ジャーナル | Blood |
| Abstract |
Hematopoietic stem/progenitor cells (HSPCs) are released from the bone marrow to the circulation by the cytokine, granulocyte colony-stimulating factor, via sympathetic nervous system (SNS)-mediated osteoblast suppression. Because the orientation of HSPCs in their osteoblastic niche is reported to be guided by [Ca(2+)], we speculated on a cooperation between the calcium-regulating hormones and SNS in the regulation of HSPC trafficking. Here, we present the severe impairment of granulocyte colony-stimulating factor-induced osteoblast suppression and subsequent HSPC mobilization in vitamin D receptor (VDR)-deficient mice. In osteoblasts, functional VDR possessing, at least in part, a transcriptional activity, was specifically induced by β2-adrenergic receptor (AR) agonists. While β2-AR agonists transiently increased mRNA expression of Vdr and its downstream gene, Rankl, 1α,25-dihydroxyvitamin-D(3) sustained the β2-AR-induced Rankl expression at high level by stabilizing VDR protein. These data suggest that VDR is essential for durable β2-AR signaling in the stem cell niche. Our study demonstrates not only a novel function of VDR as a critical modulator of HSPC trafficking, but also the presence of a SNS-mediated, bone-remodeling mechanism through VDR. VDR contributes to brain-bone-blood integration in an unanticipated way distinct from other classical calcium-regulating hormones. |
| 巻・号 | 116(25) |
| ページ | 5528-35 |
| 公開日 | 2010-12-16 |
| DOI | 10.1182/blood-2010-04-279216 |
| PII | S0006-4971(20)60348-2 |
| PMID | 20813899 |
| MeSH | Adrenergic Agonists / pharmacology Animals Blotting, Western Calcium / administration & dosage Cell Movement Chemokine CXCL12 / metabolism Enzyme-Linked Immunosorbent Assay Female Flow Cytometry Fluorescent Antibody Technique Granulocyte Colony-Stimulating Factor / administration & dosage Hematopoietic Stem Cell Mobilization Hematopoietic Stem Cells / physiology* Leukocyte Common Antigens / physiology Male Mice Mice, Inbred C57BL Mice, Knockout Osteoblasts / cytology Osteoblasts / metabolism* RANK Ligand / genetics RANK Ligand / metabolism RNA, Messenger / genetics Receptors, Adrenergic, beta-1 / chemistry Receptors, Calcitriol / physiology* Reverse Transcriptase Polymerase Chain Reaction Signal Transduction Stem Cell Niche / physiology* Sympathetic Nervous System / metabolism* Vitamin D / analogs & derivatives Vitamin D / pharmacology |
| IF | 17.794 |
| 引用数 | 35 |
| WOS 分野 | HEMATOLOGY |
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| 最多言及媒体 | Blogs |
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| リソース情報 | |
| ヒト・動物細胞 | MC3T3-E1(RCB1126) |