RRC ID 3816
Author Kasai F, Yoshihara M, Matsukuma S, O'Brien P, Ferguson-Smith MA.
Title Emergence of complex rearrangements at translocation breakpoints in a transgenic mouse; implications for mechanisms involved in the formation of chromosome rearrangements.
Journal Cytogenet. Genome Res.
Abstract Cryptic complex rearrangements as a result of a reciprocal chromosome translocation have been characterised in a transgenic mouse strain. Analysis of the breakpoint junctions in our previous studies showed that the ada transgene was integrated at the breakpoint forming a fusion gene with Golga3 (Mea2). In this study, further detailed analysis around the translocation junctions revealed that the surrounding regions were composed of 13 fragments of defined transgenic chromosome origins over approximately 1.9-Mb areas. Exactly the same cluster structure of these 13 breakpoint fragments already existed in the second generation of the transgenic mice. Our results show that this highly complex rearrangement has been conserved as the incipient form without any additional changes for 18 years up to the present generation, suggesting simultaneous occurrence of multiple events in the founder mouse.
Volume 119(1-2)
Pages 83-90
Published 2007
DOI 10.1159/000109623
PII 000109623
PMID 18160786
MeSH Animals Autoantigens / genetics Base Sequence Chromosome Breakage* Chromosomes / genetics Cytochrome P-450 Enzyme System / genetics Cytochrome P-450 Enzyme System / metabolism DNA / genetics Gene Deletion Gene Rearrangement / genetics* Membrane Proteins / genetics Mice Mice, Transgenic Multigene Family Sequence Alignment
IF 1.587
Times Cited 5
DNA material MSM Mouse BAC (RDB04214) MSMg01-121H09 MSMg01-379H11 MSMg01-090M04 MSMg01-528J16 MSMg01-412N17 MSMg01-342I20.