Reference - Detail
|Author||Kasai F, Yoshihara M, Matsukuma S, O'Brien P, Ferguson-Smith MA.|
|Title||Emergence of complex rearrangements at translocation breakpoints in a transgenic mouse; implications for mechanisms involved in the formation of chromosome rearrangements.|
|Journal||Cytogenet. Genome Res.|
Cryptic complex rearrangements as a result of a reciprocal chromosome translocation have been characterised in a transgenic mouse strain. Analysis of the breakpoint junctions in our previous studies showed that the ada transgene was integrated at the breakpoint forming a fusion gene with Golga3 (Mea2). In this study, further detailed analysis around the translocation junctions revealed that the surrounding regions were composed of 13 fragments of defined transgenic chromosome origins over approximately 1.9-Mb areas. Exactly the same cluster structure of these 13 breakpoint fragments already existed in the second generation of the transgenic mice. Our results show that this highly complex rearrangement has been conserved as the incipient form without any additional changes for 18 years up to the present generation, suggesting simultaneous occurrence of multiple events in the founder mouse.
|MeSH||Animals Autoantigens / genetics Base Sequence Chromosome Breakage* Chromosomes / genetics Cytochrome P-450 Enzyme System / genetics Cytochrome P-450 Enzyme System / metabolism DNA / genetics Gene Deletion Gene Rearrangement / genetics* Membrane Proteins / genetics Mice Mice, Transgenic Multigene Family Sequence Alignment|
|WOS Category||GENETICS & HEREDITY CELL BIOLOGY|
|DNA material||MSM Mouse BAC (RDB04214) MSMg01-121H09 MSMg01-379H11 MSMg01-090M04 MSMg01-528J16 MSMg01-412N17 MSMg01-342I20.|