論文 - 詳細
| RRC ID | 38162 |
|---|---|
| 著者 | Nakamura T, Tamura A, Murotani H, Oishi M, Jinji Y, Matsuishi K, Nagasaki Y. |
| タイトル | Large payloads of gold nanoparticles into the polyamine network core of stimuli-responsive PEGylated nanogels for selective and noninvasive cancer photothermal therapy. |
| ジャーナル | Nanoscale |
| Abstract |
A biocompatible photothermal nanomedicine based on a PEGylated nanogel containing gold nanoparticles (GNPs) in a cross-linked network core of stimuli-responsive poly[2-(N,N-diethylamino)ethyl methacrylate] (PEAMA) gel for cancer photothermal therapy (PTT) was prepared through the reduction of Au(iii) ions without any reducing agents. The influence of the reduction conditions, such as pH, temperature, and N/Au ratio (molar ratio of the amino groups in the PEGylated nanogel to the Au(iii) ions), on the formation of the GNPs in the stimuli-responsive PEAMA gel core (reducing environment) was also studied. Note that the PEGylated nanogel containing GNPs prepared at pH 6, 60 degrees C and N/Au = 1 (PEGylated GNG (1)) was found to have the highest GNP-loading capacity with a diameter of about 8 nm, as observed by TEM; viz., about 27 GNPs formed in a single PEAMA gel core. PEGylated GNG (1) showed a remarkable photothermal efficacy (DeltaT = 7.7 degrees C) under irradiation with Ar ion (Ar(+)) laser (514.5 nm) at a fluence of 39 W cm(-2) for 6 min (14 kJ cm(-2)). Note that PEGylated GNG (1) showed non-cytotoxicity in the absence of irradiation with Ar(+) laser (480 microg mL(-1): > 90% cell viability), whereas pronounced cytotoxicity (IC(50) = 110 microg mL(-1)) was observed for PEGylated GNG (1) under irradiation with Ar(+) laser at a fluence of 26 W cm(-2) for 5 min (7.8 kJ cm(-2)), because of the heat-generation from the GNPs in the cells, which resulted in selective and noninvasive cancer PTT. Thus, PEGylated GNG (1), which has a high GNP-loading capacity, would be a promising nanomedicine for cancer PTT. |
| 巻・号 | 2(5) |
| ページ | 739-46 |
| 公開日 | 2010-5-1 |
| DOI | 10.1039/b9nr00329k |
| PMID | 20648319 |
| MeSH | Gold / chemistry* HeLa Cells Humans Hydrogen-Ion Concentration Metal Nanoparticles / chemistry* Metal Nanoparticles / toxicity Metal Nanoparticles / ultrastructure Methacrylates / chemistry Nanogels Neoplasms / therapy* Nylons / chemistry Oxidation-Reduction Polyamines / chemistry* Polyethylene Glycols / chemistry* Polyethylene Glycols / toxicity Polyethyleneimine / chemistry* Polyethyleneimine / toxicity Spectrophotometry, Ultraviolet Temperature |
| IF | 6.895 |
| 引用数 | 41 |
| WOS 分野 | PHYSICS, APPLIED MATERIALS SCIENCE, MULTIDISCIPLINARY CHEMISTRY, MULTIDISCIPLINARY NANOSCIENCE & NANOTECHNOLOGY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 各媒体での言及数の合計 | 0 |
| リソース情報 | |
| ヒト・動物細胞 | HeLa(RCB0007) |