論文 - 詳細
| RRC ID | 38171 |
|---|---|
| 著者 | Sugiura S, Hattori K, Kanamori T. |
| タイトル | Microfluidic serial dilution cell-based assay for analyzing drug dose response over a wide concentration range. |
| ジャーナル | Anal Chem |
| Abstract |
In this paper we report a perfusion culture microchamber array chip with a serial dilution microfluidic network for analyzing drug dose response over a concentration range spanning 6 orders of magnitude, which is required for practical drug discovery applications. The microchamber array chip was equipped with a pressure-driven interface, in which medium and drug solution were added with a micropipet and delivered into the microfluidic network by pneumatic pressure. We demonstrated that the microchamber array chip could be used to estimate the 50% growth inhibitory concentration using the model anticancer drug paclitaxel and the model cancer cell line HeLa. The results obtained by using the microchamber array chip were consistent with those obtained by a conventional assay using microplates. The microchamber array chip, with its simple interface and well-designed microfluidic network, has potential as an efficient platform for high-throughput dose response assays in drug discovery applications. |
| 巻・号 | 82(19) |
| ページ | 8278-82 |
| 公開日 | 2010-10-1 |
| DOI | 10.1021/ac1017666 |
| PMID | 20822164 |
| MeSH | Antineoplastic Agents, Phytogenic / pharmacology* Dose-Response Relationship, Drug HeLa Cells High-Throughput Screening Assays Humans Microfluidic Analytical Techniques / methods* Paclitaxel / pharmacology* |
| IF | 6.785 |
| 引用数 | 55 |
| WOS 分野 | CHEMISTRY, ANALYTICAL |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | Patent(IFI CLAIMS) |
| 各媒体での言及数の合計 | 7 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| ヒト・動物細胞 | HeLa(RCB0007) |