論文 - 詳細
| RRC ID | 38172 |
|---|---|
| 著者 | Imura Y, Sato K, Yoshimura E. |
| タイトル | Micro total bioassay system for ingested substances: assessment of intestinal absorption, hepatic metabolism, and bioactivity. |
| ジャーナル | Anal Chem |
| Abstract |
Oral medicines and food constituents are absorbed in the intestine and metabolized in the liver, after which they exhibit their activity toward a target tissue. Micromodels of human tissues were developed to mimic these processes and bioactivities. By integrating the micromodels, we realized a micro total bioassay system for oral substances; this system comprised a microintestine, microliver, and the target components. The microchip was composed of a slide glass and polydimethylsiloxane (PDMS) sheets with microchannels fabricated by photolithography. Caco-2 cells were cultured in the intestine component, and HepG2 cells, in the liver component. The human breast carcinoma MCF-7 cells were cultured in the target component, and the activities of anticancer agents and estrogen-like substances were successfully assayed. By using this system, the overall properties of the ingested cyclophosphamide, epirubicin, 17-β estradiol, and soy isoflavone, i.e., their intestinal absorption, hepatic metabolism, and bioactivity toward target cells, could be assayed with operative ease. Further, the assay time and cell consumption were reduced compared to those in conventional in vitro bioassay systems. |
| 巻・号 | 82(24) |
| ページ | 9983-8 |
| 公開日 | 2010-12-15 |
| DOI | 10.1021/ac100806x |
| PMID | 21090751 |
| MeSH | Antineoplastic Agents / pharmacology Cell Line, Tumor Cyclophosphamide / pharmacology Epirubicin / pharmacology Estradiol / pharmacology Humans Intestinal Absorption* Isoflavones / pharmacology Lab-On-A-Chip Devices Liver / metabolism* Microchip Analytical Procedures / methods* Models, Biological* |
| IF | 6.785 |
| 引用数 | 95 |
| WOS 分野 | CHEMISTRY, ANALYTICAL |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | Patent(IFI CLAIMS) |
| 各媒体での言及数の合計 | 25 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| ヒト・動物細胞 | CACO-2(RCB0988) HepG2(RCB1648) MCF7(RCB1904) |