| RRC ID |
38222
|
| 著者 |
Ohsaki Y, Shirakawa H, Miura A, Giriwono PE, Sato S, Ohashi A, Iribe M, Goto T, Komai M.
|
| タイトル |
Vitamin K suppresses the lipopolysaccharide-induced expression of inflammatory cytokines in cultured macrophage-like cells via the inhibition of the activation of nuclear factor κB through the repression of IKKα/β phosphorylation.
|
| ジャーナル |
J Nutr Biochem
|
| Abstract |
Vitamin K is essential for blood coagulation and bone metabolism in mammals. This vitamin functions as a cofactor in the posttranslational synthesis of γ-carboxyglutamic acid (Gla) from glutamic acid residues. However, other functions of vitamin K have been reported recently. We previously found that vitamin K suppresses the inflammatory reaction induced by lipopolysaccharide (LPS) in rats and human macrophage-like THP-1 cells. In this study, we further investigated the mechanism underlying the anti-inflammatory effect of vitamin K by using cultures of LPS-treated human- and mouse-derived cells. All the vitamin K analogues analyzed in our study exhibited varied levels of anti-inflammatory activity. The isoprenyl side chain structures, except geranylgeraniol, of these analogues did not show such activity; warfarin did not interfere with this activity. The results of our study suggest that the 2-methyl-1,4-naphtoquinone ring structure contributes to express the anti-inflammatory activity, which is independent of the Gla formation activity of vitamin K. Furthermore, menaquinone-4, a form of vitamin K₂, reduced the activation of nuclear factor κB (NFκB) and inhibited the phosphorylation of IKKα/β after treatment of cells with LPS. These results clearly show that the anti-inflammatory activity of vitamin K is mediated via the inactivation of the NFκB signaling pathway.
|
| 巻・号 |
21(11)
|
| ページ |
1120-6
|
| 公開日 |
2010-11-1
|
| DOI |
10.1016/j.jnutbio.2009.09.011
|
| PII |
S0955-2863(09)00211-3
|
| PMID |
20149620
|
| MeSH |
1-Carboxyglutamic Acid / metabolism
Animals
Anti-Inflammatory Agents / metabolism
Anti-Inflammatory Agents / pharmacology
Blotting, Western
Cell Line
Cytokines / metabolism*
Humans
I-kappa B Kinase / metabolism*
Interleukin-6 / metabolism
Lipopolysaccharides / adverse effects
Lipopolysaccharides / metabolism
Mice
Mice, Inbred C57BL
NF-kappa B / metabolism*
Phosphorylation
RNA, Messenger / metabolism
Vitamin K / metabolism
Vitamin K / pharmacology*
|
| IF |
4.873
|
| 引用数 |
63
|
|
WOS 分野
|
NUTRITION & DIETETICS
BIOCHEMISTRY & MOLECULAR BIOLOGY
|
| リソース情報 |
| ヒト・動物細胞 |
RAW 264(RCB0535) |
