| RRC ID |
38242
|
| Author |
Oyane A, Murayama M, Yamazaki A, Sogo Y, Ito A, Tsurushima H.
|
| Title |
Fibronectin-DNA-apatite composite layer for highly efficient and area-specific gene transfer.
|
| Journal |
J Biomed Mater Res A
|
| Abstract |
In 2004, Shen et al. developed a safe and efficient gene transfer system using a DNA-apatite composite layer. We have recently succeeded in improving further the gene transfer efficiency by immobilizing a cell adhesion molecule laminin, in a DNA-apatite composite layer. In this study, we showed that not only laminin but fibronectin immobilized in a DNA-apatite composite layer enhances cell adhesion and cell spreading on the layer, thereby markedly improving the gene transfer efficiency. Therefore, the immobilization of a cell adhesion molecule in a DNA-apatite composite layer is crucial for improving the gene transfer efficiency. By using fibronectin instead of laminin and optimizing the condition to prepare the fibronectin-DNA-apatite composite layer, the amount (weight) of cell adhesion molecule required was reduced to approximately one-fourth while retaining the relatively high gene transfer efficiency. It was also shown that the resulting fibronectin-DNA-apatite composite layer prepared under the optimized condition mediated the area-specific gene transfer on its surface, that is, DNA was preferentially transferred to the cells adhering to the surface of the fibronectin-DNA-apatite composite layer. The present gene transfer system with potential for area-specific transfection and advantages of safety and relatively high efficiency would be useful in tissue engineering applications, gene therapy, and production of transfection microarrays.
|
| Volume |
92(3)
|
| Pages |
1038-47
|
| Published |
2010-3-1
|
| DOI |
10.1002/jbm.a.32449
|
| PMID |
19296545
|
| MeSH |
Animals
Apatites*
CHO Cells
Cricetinae
Cricetulus
DNA / chemistry*
Fibronectins / chemistry*
Gene Transfer Techniques*
Microscopy, Electron, Scanning
X-Ray Diffraction
|
| IF |
3.525
|
| Times Cited |
23
|
|
WOS Category
|
ENGINEERING, BIOMEDICAL
MATERIALS SCIENCE, BIOMATERIALS
|
| Resource |
| Human and Animal Cells |
CHO-K1 |
