Reference - Detail
| RRC ID | 38242 |
|---|---|
| Author | Oyane A, Murayama M, Yamazaki A, Sogo Y, Ito A, Tsurushima H. |
| Title | Fibronectin-DNA-apatite composite layer for highly efficient and area-specific gene transfer. |
| Journal | J Biomed Mater Res A |
| Abstract |
In 2004, Shen et al. developed a safe and efficient gene transfer system using a DNA-apatite composite layer. We have recently succeeded in improving further the gene transfer efficiency by immobilizing a cell adhesion molecule laminin, in a DNA-apatite composite layer. In this study, we showed that not only laminin but fibronectin immobilized in a DNA-apatite composite layer enhances cell adhesion and cell spreading on the layer, thereby markedly improving the gene transfer efficiency. Therefore, the immobilization of a cell adhesion molecule in a DNA-apatite composite layer is crucial for improving the gene transfer efficiency. By using fibronectin instead of laminin and optimizing the condition to prepare the fibronectin-DNA-apatite composite layer, the amount (weight) of cell adhesion molecule required was reduced to approximately one-fourth while retaining the relatively high gene transfer efficiency. It was also shown that the resulting fibronectin-DNA-apatite composite layer prepared under the optimized condition mediated the area-specific gene transfer on its surface, that is, DNA was preferentially transferred to the cells adhering to the surface of the fibronectin-DNA-apatite composite layer. The present gene transfer system with potential for area-specific transfection and advantages of safety and relatively high efficiency would be useful in tissue engineering applications, gene therapy, and production of transfection microarrays. |
| Volume | 92(3) |
| Pages | 1038-47 |
| Published | 2010-3-1 |
| DOI | 10.1002/jbm.a.32449 |
| PMID | 19296545 |
| MeSH | Animals Apatites* CHO Cells Cricetinae Cricetulus DNA / chemistry* Fibronectins / chemistry* Gene Transfer Techniques* Microscopy, Electron, Scanning X-Ray Diffraction |
| IF | 3.525 |
| Times Cited | 23 |
| WOS Category | ENGINEERING, BIOMEDICAL MATERIALS SCIENCE, BIOMATERIALS |
| Altmetric score |
オルトメトリクス指標項目
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| Total number of mentions | 0 |
| Resource | |
| Human and Animal Cells | CHO-K1 |