論文 - 詳細
| RRC ID | 38253 |
|---|---|
| 著者 | Sato Y, Takata H, Kobayashi N, Nagata S, Nakagata N, Ueno T, Takiguchi M. |
| タイトル | Failure of effector function of human CD8+ T Cells in NOD/SCID/JAK3⁻/⁻ immunodeficient mice transplanted with human CD34+ hematopoietic stem cells. |
| ジャーナル | PLoS One |
| Abstract |
Humanized mice, which are generated by transplanting human CD34+ hematopoietic stem cells into immunodeficient mice, are expected to be useful for the research on human immune responses. It is reported that antigen-specific T cell responses occur in immunodeficient mice transplanted with both human fetal thymus/liver tissues and CD34+ fetal cells, but it remains unclear whether antigen-specific T cell responses occur in those transplanted with only human CD34+ hematopoietic stem cells (HSCs). Here we investigated the differentiation and function of human CD8+ T cells reconstituted in NOD/SCID/Jak3⁻/⁻ mice transplanted with human CD34+ HSCs (hNOK mice). Multicolor flow cytometric analysis demonstrated that human CD8+ T cells generated from the CD34+ HSCs comprised only 3 subtypes, i.e., CD27(high)CD28+CD45RA+CCR7+, CD27+CD28+CD45RA⁻CCR7+, and CD27+CD28+CD45RA⁻CCR7⁻and had 3 phenotypes for 3 lytic molecules, i.e., perforin(Per)⁻granzymeA(GraA)⁻granzymeB(GraB)⁻, Per⁻GraA+GraB⁻, and Per(low)GraA+GraB+. These CD8+ T cells failed to produce IFN-γ and to proliferate after stimulation with alloantigens. These results indicate that the antigen-specific T cell response cannot be elicited in mice transplanted with only human CD34+ HSCs, because the T cells fail to develop normally in such mice. |
| 巻・号 | 5(10) |
| 公開日 | 2010-10-1 |
| DOI | 10.1371/journal.pone.0013109 |
| PII | e13109 |
| PMID | 20957041 |
| PMC | PMC2948507 |
| MeSH | Animals Antigens, CD34 / immunology* CD8-Positive T-Lymphocytes / immunology* Cytokines / biosynthesis Flow Cytometry Hematopoietic Stem Cell Transplantation* Humans Immunophenotyping Janus Kinase 3 / genetics* Mice Mice, Inbred NOD Mice, SCID |
| IF | 2.74 |
| 引用数 | 9 |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | Patent(IFI CLAIMS) |
| 各媒体での言及数の合計 | 1 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| 研究用ヒト臍帯血幹細胞 | |