| RRC ID |
38256
|
| 著者 |
Mueller I, Kobayashi R, Nakajima T, Ishii M, Ogawa K.
|
| タイトル |
Effective and steady differentiation of a clonal derivative of P19CL6 embryonal carcinoma cell line into beating cardiomyocytes.
|
| ジャーナル |
J Biomed Biotechnol
|
| Abstract |
The P19CL6 cell line is a useful model to study cardiac differentiation in vitro. However, large variations were noticed in the differentiation rates among previous reports as well as our individual experiments. To overcome the unstable differentiation, we established P19CL6-A1, a new clonal derivative of P19CL6 that could differentiate into cardiomyocytes more efficiently and stably than the parent using the double stimulation with 5-Aza and DMSO based on the previous report. We also introduced a new software, Visorhythm, that can analyze the temporal variations in the beating rhythms and can chart correlograms displaying the oscillated rhythms. Using P19CL6-A1-derived cardiomyocytes and the software, we demonstrated that the correlograms could clearly display the enhancement of beating rates by cardiotonic reagents. These indicate that a combination of P19CL6-A1 and Visorhythm is a useful tool that can provide invaluable assistance in inotropic drug discovery, drug screening, and toxicity testing.
|
| 巻・号 |
2010
|
| ページ |
380561
|
| 公開日 |
2010-1-1
|
| DOI |
10.1155/2010/380561
|
| PMID |
20368798
|
| PMC |
PMC2846686
|
| MeSH |
1-Methyl-3-isobutylxanthine / pharmacology
Animals
Cell Differentiation / physiology*
Cell Line, Tumor
Dose-Response Relationship, Drug
Gene Expression Profiling / methods
Mice
Mice, Inbred BALB C
Microscopy, Fluorescence
Microscopy, Phase-Contrast
Myocytes, Cardiac / cytology
Myocytes, Cardiac / drug effects
Myocytes, Cardiac / physiology*
RNA, Messenger
Reverse Transcriptase Polymerase Chain Reaction
|
| 引用数 |
11
|
| リソース情報 |
| ヒト・動物細胞 |
|
